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However, the lack of rapid, efficient, and high\u2010throughput techniques for urinary exosome enrichment limits their clinical application. This study presents an exosome cyclic enrichment chip based on asymmetric impinging streams and a metal\u2010organic framework (MOF)\u2010on\u2010MOF material. The newly synthesized MOF material exhibited excellent exosome\u2010enrichment capabilities. Moreover, the integration of asymmetric impinging streams and a cyclic fluidic pathway within the chip notably enhanced the collision efficiency between exosomes and MOF. Leveraging the chip, enables the rapid and efficient enrichment of exosomes from a 10\u00a0mL urine sample within 30\u00a0min, without the requirement for sample pre\u2010concentration. In a clinical study of 300 subjects from two centers, a BCa diagnostic model based on urinary exosomal NORAD (Exo\u2010NORAD) and urine sediment NORAD (Sed\u2010NORAD) is developed, which showed a higher detection positivity rate than BCa fluorescence in situ hybridization (FISH) and urinary cytology. Exo\u2010NORAD is identified as an independent prognostic factor for BCa using Cox regression analysis. In conclusion, this study provides a new approach for the noninvasive diagnosis and prognosis prediction of BCa.<\/jats:p>","DOI":"10.1002\/adhm.202500848","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T01:34:25Z","timestamp":1745890465000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cyclic Enrichment of Urinary Exosomes Using a MOF\u2010on\u2010MOF\u2010Based Asymmetric Impinging Streams Chip for Bladder Cancer Diagnosis and Prognosis Prediction"],"prefix":"10.1002","volume":"14","author":[{"given":"Min","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Zhongnan Hospital of Wuhan University  Wuhan 430071 China"},{"name":"Hubei Provincial Clinical Research Center for Molecular Diagnostics  Wuhan 430071 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaihua","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Zhongnan Hospital of Wuhan University  Wuhan 430071 China"},{"name":"Hubei Provincial Clinical Research Center for Molecular Diagnostics  Wuhan 430071 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyu","family":"Meng","sequence":"additional","affiliation":[{"name":"Health Science Center Hubei Minzu University  Enshi 445000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Rong","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Zhongnan Hospital of Wuhan University  Wuhan 430071 China"},{"name":"Hubei Provincial Clinical Research Center for Molecular Diagnostics  Wuhan 430071 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Xia","sequence":"additional","affiliation":[{"name":"National \u201c111\u201d Center for Cellular Regulation and Molecular Pharmaceutics School of Life and Health Sciences Hubei University of Technology  Wuhan 430068 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Zhongnan Hospital of Wuhan University  Wuhan 430071 China"},{"name":"Hubei Provincial Clinical Research Center for Molecular Diagnostics  Wuhan 430071 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"He","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Zhongnan Hospital of Wuhan University  Wuhan 430071 China"},{"name":"Hubei Provincial Clinical Research Center for Molecular Diagnostics  Wuhan 430071 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiurong","family":"He","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Zhongnan Hospital of Wuhan University  Wuhan 430071 China"},{"name":"Hubei Provincial Clinical Research Center for Molecular Diagnostics  Wuhan 430071 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjie","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Biological Repositories Zhongnan Hospital of Wuhan University  Wuhan 430071 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongzu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology Zhongnan Hospital of Wuhan University  169 Donghu Road Wuhan 430071 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 430030 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhou","sequence":"additional","affiliation":[{"name":"Guangzhou Anfang Biotechnology Co., Ltd.  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