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Here, it is reported that GBM cell lysate nanovaccines boosted with TLR9 agonist CpG ODN (GlioVac) via a strategic vaccination regimen achieve complete regression of malignant murine GBM tumors. Subcutaneous administration of GlioVac promotes uptake by cervical lymph nodes and antigen presentation cells, bolstering antigen cross\u2010presentation and infiltration of GBM\u2010specific CD8<jats:sup>+<\/jats:sup> T cells into the tumor. Notably, a regimen involving two subcutaneous and three intravenous vaccinations not only activates systemic anti\u2010GBM immunity but also further enhances the tumor infiltration of cytotoxic T lymphocytes, effectively reshaping the \u201ccold\u201d GBM tumor into a \u201chot\u201d tumor. This approach led to a state of tumor\u2010free survival in 5 out of 7 mice bearing the established GL261 GBM model with complete protection from tumor rechallenge. In an orthotopic hRas\u2010GBM model induced by a lentiviral plasmid, GlioVac resulted in \u2248100% complete tumor regression. These findings suggest that GlioVac provides a personalized therapeutic vaccine strategy for glioblastoma.<\/jats:p>","DOI":"10.1002\/adhm.202500911","type":"journal-article","created":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:34:40Z","timestamp":1745469280000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Glioblastoma Cell Lysate and Adjuvant Nanovaccines via Strategic Vaccination Completely Regress Established Murine Tumors"],"prefix":"10.1002","volume":"14","author":[{"given":"Songsong","family":"Zhao","sequence":"first","affiliation":[{"name":"Biomedical Polymers Laboratory College of Chemistry Chemical Engineering and Materials Science and State Key Laboratory of Radiation Medicine and Protection Soochow University  Suzhou 215123 P. R. 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