{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T19:47:01Z","timestamp":1792093621673,"version":"4.4.0"},"reference-count":36,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T00:00:00Z","timestamp":1752019200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008005","name":"Yonsei University College of Medicine","doi-asserted-by":"publisher","award":["6\u20102021\u20100005"],"award-info":[{"award-number":["6\u20102021\u20100005"]}],"id":[{"id":"10.13039\/501100008005","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003625","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["HV20C0205"],"award-info":[{"award-number":["HV20C0205"]}],"id":[{"id":"10.13039\/501100003625","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003655","name":"National Institute of Food and Drug Safety Evaluation","doi-asserted-by":"publisher","award":["23202MFDS151"],"award-info":[{"award-number":["23202MFDS151"]}],"id":[{"id":"10.13039\/501100003655","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2021R1C1C1008626"],"award-info":[{"award-number":["2021R1C1C1008626"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human papillomavirus (HPV) vaccines have substantially reduced cervical cancer and other HPV\u2010related diseases in high\u2010income countries, with male vaccination addressing transmission as well as head and neck cancers. However, in low\u2010 and middle\u2010income countries, widespread vaccination efforts are hindered by considerable costs, insufficient medical staff, and challenges in maintaining vaccine stability. Conventional microneedle vaccine production often compromises the structural integrity of virus\u2010like particles (VLPs), thereby reducing their effectiveness. To address this challenge, a dissolving microneedle platform is developed that preserves VLP stability for over six months. Administration of this vaccine platform to buccal tissue enables effective antigen delivery, inducing T follicular helper (Tfh) cell differentiation, germinal center formation, and the production of high\u2010titer neutralizing antibodies. Buccal application also conferred sterilizing immunity in both buccal and vaginal tissues. Moreover, the vaccine induced durable immune memory, with memory B cells and plasma cells persisting in buccal tissue and bone marrow for six months. This innovation addresses critical barriers in resource\u2010limited regions by enhancing VLP stability and accessibility. Furthermore, the platform's applicability extends to other vaccines requiring structural integrity for efficacy, representing a transformative approach to global immunization efforts.<\/jats:p>","DOI":"10.1002\/adhm.202500963","type":"journal-article","created":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T05:20:03Z","timestamp":1752124803000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Dissolving Microneedle for Maintaining the Integrity of HPV Virus\u2010Like Particles Enabling Durable Sterile Protection Across Various Mucosal Tissues"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-4787-2716","authenticated-orcid":false,"given":"Hyemi","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology Yonsei University College of Medicine  50\u20131 Yonsei\u2010ro, Seodaemun\u2010gu Seoul 03722 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inhyuk","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Yonsei University College of Medicine  50\u20131 Yonsei\u2010ro, Seodaemun\u2010gu Seoul 03722 Republic of Korea"},{"name":"Brain Korea 21 Project for Medical Science Yonsei University College of Medicine  50\u20131 Yonsei\u2010ro, Seodaemun\u2010gu Seoul 03722 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juhee","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Yonsei University College of Medicine  50\u20131 Yonsei\u2010ro, Seodaemun\u2010gu Seoul 03722 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaiwon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Yonsei University College of Medicine  50\u20131 Yonsei\u2010ro, Seodaemun\u2010gu Seoul 03722 Republic of Korea"},{"name":"Brain Korea 21 Project for Medical Science Yonsei University College of Medicine  50\u20131 Yonsei\u2010ro, Seodaemun\u2010gu Seoul 03722 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In\u2010Jeong","family":"Choi","sequence":"additional","affiliation":[{"name":"QuadMedicine R&amp;D Centre QuadMedicine, Inc.  sagimakgol\u2010ro, 45beon\u2010gil, jungwon\u2010gu seongnam\u2010si Gyeonggi\u2010do 13209 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miran","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Yonsei University College of Medicine  50\u20131 Yonsei\u2010ro, Seodaemun\u2010gu Seoul 03722 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min\u2010Seok","family":"Rha","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Yonsei University College of Medicine  50\u20131 Yonsei\u2010ro, Seodaemun\u2010gu Seoul 03722 Republic of Korea"},{"name":"The Airway Mucus Institute Yonsei University College of Medicine  50\u20131 Yonsei\u2010ro, Seodaemun\u2010gu Seoul 03722 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youngjae","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Yonsei University College of Medicine  50\u20131 Yonsei\u2010ro, Seodaemun\u2010gu Seoul 03722 Republic of Korea"},{"name":"Brain Korea 21 Project for Medical Science Yonsei University College of Medicine  50\u20131 Yonsei\u2010ro, Seodaemun\u2010gu Seoul 03722 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4846-6259","authenticated-orcid":false,"given":"Jooyoung","family":"Kim","sequence":"additional","affiliation":[{"name":"QuadMedicine R&amp;D Centre QuadMedicine, Inc.  sagimakgol\u2010ro, 45beon\u2010gil, jungwon\u2010gu seongnam\u2010si Gyeonggi\u2010do 13209 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung\u2010Ki","family":"Baek","sequence":"additional","affiliation":[{"name":"QuadMedicine R&amp;D Centre QuadMedicine, Inc.  sagimakgol\u2010ro, 45beon\u2010gil, jungwon\u2010gu seongnam\u2010si Gyeonggi\u2010do 13209 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4684-7568","authenticated-orcid":false,"given":"Jung\u2010Hwan","family":"Park","sequence":"additional","affiliation":[{"name":"Department of BioNano Technology Gachon University  1342, Seongnam\u2010daero, Sujeong\u2010gu Seongnam\u2010si Gyeonggi\u2010do 13120 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyung\u2010Ju","family":"Cho","sequence":"additional","affiliation":[{"name":"Brain Korea 21 Project for Medical Science Yonsei University College of Medicine  50\u20131 Yonsei\u2010ro, Seodaemun\u2010gu Seoul 03722 Republic of Korea"},{"name":"Department of Otorhinolaryngology Yonsei University College of Medicine  50\u20131 Yonsei\u2010ro, Seodaemun\u2010gu Seoul 03722 Republic of Korea"},{"name":"The Airway Mucus Institute Yonsei University College of Medicine  50\u20131 Yonsei\u2010ro, Seodaemun\u2010gu Seoul 03722 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7386-9589","authenticated-orcid":false,"given":"Kihyuck","family":"Kwak","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Yonsei University College of Medicine  50\u20131 Yonsei\u2010ro, Seodaemun\u2010gu Seoul 03722 Republic of Korea"},{"name":"Brain Korea 21 Project for Medical Science Yonsei University College of Medicine  50\u20131 Yonsei\u2010ro, Seodaemun\u2010gu Seoul 03722 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,9]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djh104"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.b3884"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acap.2017.09.014"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2021.08.043"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2021.26.50.2001659"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)02178-4"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.04.106"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200425547"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2017.12.079"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2868"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-023-01597-9"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/v15051109"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.790121"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2024.126399"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2013.06.016"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1150238"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2024.1380663"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(14)71073-4"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2023.10.062"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(12)70187-1"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.200701205"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2021.05.102"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2015.11.019"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2021.08.024"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.07.019"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.12.048"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6603695"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2023.114904"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11081165"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-020-00236-w"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2011.07.006"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.953088"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-021-00583-2"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics15030744"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2020.10.002"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0097232"}],"updated-by":[{"DOI":"10.1002\/adhm.70629","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T00:00:00Z","timestamp":1765670400000}}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202500963","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T06:29:30Z","timestamp":1758263370000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202500963"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,9]]},"references-count":36,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1002\/adhm.202500963"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202500963","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,9]]},"assertion":[{"value":"2025-02-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2500963"}}