{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T23:43:16Z","timestamp":1793317396410,"version":"4.5.0"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T00:00:00Z","timestamp":1757635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T00:00:00Z","timestamp":1757635200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Drug delivery system (DDS) is an important branch of pharmaceutics. Rational modification of the physicochemical properties of DDSs can further improve their targeting efficiency. Herein, a bionic DDS is reported for AKI that is scRNA\u2010seq\u2010guided, custom\u2010designed, and prepared, targeting the Key Cell Subtype for Pathological Progression (KCS\u2010PP) of acute kidney injury (AKI). Specifically, scRNA\u2010seq is utilized to identify a specific renal tubular epithelial cell subtype (PTIs) as the KCS\u2010PP for AKI from numerous cellular subtypes. Additionally, specific cell adhesion molecules (VCAM1 and ICAM1) are identified as the Targeting Drug Delivery Mediators (TDDMs) for PTIs from a list of 1000 marker genes of PTIs. Based on this progress, PTI\u2010targeting bionic DDS, named BRNCs@AMMOs is custom\u2010designed and prepared, and used them for AKI treatment in vitro and in vivo. In vitro, BRNCs@AMMOs shows that its adhesion ability in PTI model cells is 3.2 times that in normal cells. In vivo, 6 h after renal pelvis injection, the MFI of BRNCs@AMMOs\u2010DiI in AKI kidneys is 3.7 times that of sham kidneys. The findings demonstrate that BRNCs@AMMOs exhibits prolonged retention in PTI model cells and AKI kidneys. Overall, the custom\u2010designed and prepared PTI\u2010targeting bionic DDS, may promote the customized design and preparation of DDSs for different diseases and targets, is reported.<\/jats:p>","DOI":"10.1002\/adhm.202501497","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T07:12:48Z","timestamp":1757661168000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Customized Design and Preparation of Bionic Drug Delivery System Leveraging Single\u2010Cell RNA Sequencing for Precisely Targeted Therapy"],"prefix":"10.1002","volume":"15","author":[{"given":"Tong","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Pharmacy, The First People's Hospital of Xiaoshan District Xiaoshan Affiliated Hospital of Wenzhou Medical University  Hangzhou 311200 P. R. 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