{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T13:50:42Z","timestamp":1790257842167,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T00:00:00Z","timestamp":1755475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52272279"],"award-info":[{"award-number":["52272279"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52072393"],"award-info":[{"award-number":["52072393"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32000543"],"award-info":[{"award-number":["32000543"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82272104"],"award-info":[{"award-number":["82272104"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017610","name":"Shenzhen Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["ZDSYS20220606100606013"],"award-info":[{"award-number":["ZDSYS20220606100606013"]}],"id":[{"id":"10.13039\/501100017610","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017610","name":"Shenzhen Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["ZDSYS20220606100803007"],"award-info":[{"award-number":["ZDSYS20220606100803007"]}],"id":[{"id":"10.13039\/501100017610","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017610","name":"Shenzhen Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["RCYX20221008092901004"],"award-info":[{"award-number":["RCYX20221008092901004"]}],"id":[{"id":"10.13039\/501100017610","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2024B1515020102"],"award-info":[{"award-number":["2024B1515020102"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2024A1515010500"],"award-info":[{"award-number":["2024A1515010500"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012165","name":"Key Technologies Research and Development Program","doi-asserted-by":"publisher","award":["2021YFA0909900"],"award-info":[{"award-number":["2021YFA0909900"]}],"id":[{"id":"10.13039\/501100012165","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Hydrogen sulfide (H\n                    <jats:sub>2<\/jats:sub>\n                    S)\u2010mediated gaseous therapies feature high therapeutic efficacy and biosafety in cancer treatment, but conventional H\n                    <jats:sub>2<\/jats:sub>\n                    S delivery protocols suffer from poor tumor specificity and uncontrollable release. Here, a living therapeutic biohybrid is developed that integrates engineered microbes for in situ H\n                    <jats:sub>2<\/jats:sub>\n                    S production with self\u2010mineralized copper sulfide (CuS) nanoparticles, enabling synergistic H\n                    <jats:sub>2<\/jats:sub>\n                    S gaseous\u2010photothermal cancer treatment. These engineered facultative anaerobic bacteria\u00a0\n                    <jats:italic>Vibrio natriegens<\/jats:italic>\n                    continuously produce H\n                    <jats:sub>2<\/jats:sub>\n                    S and synthesize CuS nanoparticles, forming Bac@CuS living biohybrids that inhibit the mitochondrial electron transport chain through H\n                    <jats:sub>2<\/jats:sub>\n                    S production, leading to increased reactive oxygen species production and subsequent apoptosis of cancer cells. Concurrently, Bac@CuS\u2010mediated photothermal effect induces hyperthermia, further impairing mitochondrial function and enhancing cancer\u2010cell death. In vivo studies demonstrate that Bac@CuS living biohybrids feature excellent biocompatibility and have achieved\u00a0a 95.4% tumor inhibition rate in the breast tumor\u2010bearing mouse model. The biohybrid therapeutic platform enables the engineered bacteria to produce non\u2010native effectors alongside with nanoparticles, integrating synthetic biology with nanotechnology and offering a novel approach for efficient cancer eradication.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202501621","type":"journal-article","created":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T07:15:43Z","timestamp":1755501343000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Bioengineering Living Biohybrid Therapeutics for Synergistic H\n                    <sub>2<\/sub>\n                    S Gaseous\u2010Photothermal Cancer Eradication"],"prefix":"10.1002","volume":"15","author":[{"given":"Xiaolian","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Pharmacology School of Medicine Shenzhen Campus of Sun Yat\u2010Sen University  Shenzhen 518107 P. R. China"},{"name":"Center for Materials Synthetic Biology, State Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Shenzhen 518000 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Medicine Shenzhen Campus of Sun Yat\u2010Sen University  Shenzhen 518107 P. R. China"},{"name":"Center for Materials Synthetic Biology, State Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Shenzhen 518000 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenyao","family":"Wu","sequence":"additional","affiliation":[{"name":"Materdicine Lab, School of Life Sciences Shanghai University  Shanghai 200444 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuelan","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Medicine Shenzhen Campus of Sun Yat\u2010Sen University  Shenzhen 518107 P. R. China"},{"name":"Center for Materials Synthetic Biology, State Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Shenzhen 518000 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianwei","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering National University of Singapore  Singapore 117585 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengjie","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Medicine Shenzhen Campus of Sun Yat\u2010Sen University  Shenzhen 518107 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingding","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Medicine Shenzhen Campus of Sun Yat\u2010Sen University  Shenzhen 518107 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiliang","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering National University of Singapore  Singapore 117585 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianghui","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Medicine Shenzhen Campus of Sun Yat\u2010Sen University  Shenzhen 518107 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangfang","family":"Duan","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Medicine Shenzhen Campus of Sun Yat\u2010Sen University  Shenzhen 518107 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Feng","sequence":"additional","affiliation":[{"name":"Materdicine Lab, School of Life Sciences Shanghai University  Shanghai 200444 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Chen","sequence":"additional","affiliation":[{"name":"Materdicine Lab, School of Life Sciences Shanghai University  Shanghai 200444 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0699-0351","authenticated-orcid":false,"given":"Xiang","family":"Gao","sequence":"additional","affiliation":[{"name":"Center for Materials Synthetic Biology, State Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Shenzhen 518000 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,18]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42255-023-00862-3"},{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-29284-7"},{"key":"e_1_2_8_1_3","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-024-01583-8"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2205044120"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-52006-0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-2222-9"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-024-06829-w"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2014.2305"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2013.10.023"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202008481"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.3c11328"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202201069"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2021.11.004"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.add9667"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-15508-1"},{"key":"e_1_2_8_8_3","doi-asserted-by":"publisher","DOI":"10.1038\/s44222-023-00119-4"},{"key":"e_1_2_8_8_4","doi-asserted-by":"publisher","DOI":"10.1038\/s41578-021-00350-8"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41893-023-01233-2"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2024.133622"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abo6163"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-023-01476-2"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201912824"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202405673"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3CS00655G"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2021.10.007"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-43631-2"},{"key":"e_1_2_8_15_3","doi-asserted-by":"publisher","DOI":"10.1042\/EBC20200135"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.113481"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.705583"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.9.3939-3944.2000"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42255-024-01068-x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.1c00567"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20566-6"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.7b06870"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22126452"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-022-00741-6"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202304312"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101606"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.01.030"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-023-00775-0"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2219352120"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adj6409"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-024-00979-4"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.7b04263"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202501621","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T14:42:43Z","timestamp":1768056163000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202501621"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,18]]},"references-count":44,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1002\/adhm.202501621"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202501621","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,18]]},"assertion":[{"value":"2025-03-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01621"}}