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Previously, the CRISmers system is developed, a Clustered Regularly Interspaced Short Palindromic Repeats\/CRISPR\u2010associated proteins (CRISPR\/Cas)\u2010mediated cellular screening platform for the identification of RNA aptamers. In this study, CRISmers are applied to target the fusion peptide (FP) of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS\u2010CoV\u20102), a conserved region across coronavirus genera, with <jats:italic>E.coli<\/jats:italic> serving as the screening host. From this approach, a lead aptamer, #FP\u201010 is identified, which demonstrates potent pan\u2010coronavirus neutralization activity against multiple SARS\u2010CoV\u20102 variants and alphacoronaviruses (HCoV\u2010229E and HCoV\u2010NL63), demonstrating its potential for development into a broad\u2010spectrum therapeutic candidate.<\/jats:p>","DOI":"10.1002\/adhm.202501869","type":"journal-article","created":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T09:37:28Z","timestamp":1750066648000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Pan\u2010Coronavirus Neutralizing Aptamers Through CRISmers Targeting the Conserved Fusion Peptide"],"prefix":"10.1002","volume":"14","author":[{"given":"Ju","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology Chinese Academy of Sciences  Beijing 100101 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