{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T20:14:23Z","timestamp":1787170463569,"version":"build-2736575974"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T00:00:00Z","timestamp":1759449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T00:00:00Z","timestamp":1759449600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["92268120"],"award-info":[{"award-number":["92268120"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31871295"],"award-info":[{"award-number":["31871295"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22205012"],"award-info":[{"award-number":["22205012"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82373158"],"award-info":[{"award-number":["82373158"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017610","name":"Shenzhen Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["KJZD20240903103400001"],"award-info":[{"award-number":["KJZD20240903103400001"]}],"id":[{"id":"10.13039\/501100017610","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017610","name":"Shenzhen Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["JCYJ20220531091802006"],"award-info":[{"award-number":["JCYJ20220531091802006"]}],"id":[{"id":"10.13039\/501100017610","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20211228"],"award-info":[{"award-number":["BK20211228"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Maintaining skeletal muscle mass is crucial for health, as muscle atrophy caused by drugs, cancer, or aging poses serious risks. However, there are few effective pharmacological interventions targeting muscle atrophy, highlighting the need for new therapeutic strategies. In this study, in vivo self\u2010assembled siRNA is designed to silence myostatin (MSTN), a key regulator of muscle growth and atrophy, aiming to prevent muscle atrophy. Using synthetic constructs and the host liver as a scaffold, the assembly of MSTN\u2010siRNA is guided into muscle\u2010specific peptide MSP\u2010tagged small extracellular vesicles (sEVs). These MSP\u2010tagged sEVs selectively deliver MSTN\u2010siRNA to muscle tissue. Treatment significantly reduces MSTN protein levels in skeletal muscle, promotes muscle mass gain in healthy mice, and protectes skeletal muscles from atrophy in cancer\u2010 and dexamethasone\u2010induced muscle atrophy models. Notably, the sEV\u2010encapsulated MSTN\u2010siRNA is produced in a nontoxic, nonimmunogenic, and biocompatible manner. This study offers a promising therapeutic approach for muscle atrophy, addressing a key gap in current treatment options and potentially improving outcomes for patients with muscle\u2010wasting conditions.<\/jats:p>","DOI":"10.1002\/adhm.202502186","type":"journal-article","created":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T10:58:59Z","timestamp":1759489139000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Silencing Myostatin Using In Vivo Self\u2010Assembled siRNA Protects Against Cancer\u2010 and Dexamethasone\u2010Induced Muscle Atrophy"],"prefix":"10.1002","volume":"15","author":[{"given":"Xin","family":"Yin","sequence":"first","affiliation":[{"name":"PKU\u2010HKUST ShenZhen\u2010HongKong Institution  Shenzhen Guangdong 518057 China"},{"name":"Pingshan Translational Medicine Center, Shenzhen Bay Laboratory  Shenzhen Guangdong 518118 China"},{"name":"Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy Chinese Academy of Medical Sciences Research Unit of Extracellular RNA State Key Laboratory of Pharmaceutical Biotechnology Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology NJU Advanced Institute of Life Sciences (NAILS) School of Life Sciences Nanjing University  Nanjing Jiangsu 210023 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azhar","family":"Anwar","sequence":"additional","affiliation":[{"name":"Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy Chinese Academy of Medical Sciences Research Unit of Extracellular RNA State Key Laboratory of Pharmaceutical Biotechnology Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology NJU Advanced Institute of Life Sciences (NAILS) School of Life Sciences Nanjing University  Nanjing Jiangsu 210023 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiehao","family":"Chen","sequence":"additional","affiliation":[{"name":"Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy Chinese Academy of Medical Sciences Research Unit of Extracellular RNA State Key Laboratory of Pharmaceutical Biotechnology Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology NJU Advanced Institute of Life Sciences (NAILS) School of Life Sciences Nanjing University  Nanjing Jiangsu 210023 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghao","family":"Sun","sequence":"additional","affiliation":[{"name":"The Research Center of Military Exercise Science The Army Engineering University of PLA  Nanjing Jiangsu 210007 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Likun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Tianjin Medical University Cancer Institute and Hospital National Clinical Research Center for Cancer Tianjin's Clinical Research Center for Cancer Key Laboratory of Cancer Prevention and Therapy Tianjin Key Laboratory of Digestive Cancer  Tianjin 300060 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Tang","sequence":"additional","affiliation":[{"name":"Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy Chinese Academy of Medical Sciences Research Unit of Extracellular RNA State Key Laboratory of Pharmaceutical Biotechnology Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology NJU Advanced Institute of Life Sciences (NAILS) School of Life Sciences Nanjing University  Nanjing Jiangsu 210023 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingwei","family":"Guo","sequence":"additional","affiliation":[{"name":"Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy Chinese Academy of Medical Sciences Research Unit of Extracellular RNA State Key Laboratory of Pharmaceutical Biotechnology Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology NJU Advanced Institute of Life Sciences (NAILS) School of Life Sciences Nanjing University  Nanjing Jiangsu 210023 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linbo","family":"Yan","sequence":"additional","affiliation":[{"name":"Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy Chinese Academy of Medical Sciences Research Unit of Extracellular RNA State Key Laboratory of Pharmaceutical Biotechnology Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology NJU Advanced Institute of Life Sciences (NAILS) School of Life Sciences Nanjing University  Nanjing Jiangsu 210023 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongci","family":"Chen","sequence":"additional","affiliation":[{"name":"Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy Chinese Academy of Medical Sciences Research Unit of Extracellular RNA State Key Laboratory of Pharmaceutical Biotechnology Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology NJU Advanced Institute of Life Sciences (NAILS) School of Life Sciences Nanjing University  Nanjing Jiangsu 210023 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Yin","sequence":"additional","affiliation":[{"name":"Pingshan Translational Medicine Center, Shenzhen Bay Laboratory  Shenzhen Guangdong 518118 China"},{"name":"State Key Laboratory of Chemical Oncogenomics School of Chemical Biology and Biotechnology Peking University Shenzhen Graduate School  Shenzhen 518055 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen\u2010Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy Chinese Academy of Medical Sciences Research Unit of Extracellular RNA State Key Laboratory of Pharmaceutical Biotechnology Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology NJU Advanced Institute of Life Sciences (NAILS) School of Life Sciences Nanjing University  Nanjing Jiangsu 210023 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zigang","family":"Li","sequence":"additional","affiliation":[{"name":"Pingshan Translational Medicine Center, Shenzhen Bay Laboratory  Shenzhen Guangdong 518118 China"},{"name":"State Key Laboratory of Chemical Oncogenomics School of Chemical Biology and Biotechnology Peking University Shenzhen Graduate School  Shenzhen 518055 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jizheng","family":"Ma","sequence":"additional","affiliation":[{"name":"The Research Center of Military Exercise Science The Army Engineering University of PLA  Nanjing Jiangsu 210007 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyuan","family":"Sheng","sequence":"additional","affiliation":[{"name":"PKU\u2010HKUST ShenZhen\u2010HongKong Institution  Shenzhen Guangdong 518057 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5807-4219","authenticated-orcid":false,"given":"Xi","family":"Chen","sequence":"additional","affiliation":[{"name":"Pingshan Translational Medicine Center, Shenzhen Bay Laboratory  Shenzhen Guangdong 518118 China"},{"name":"Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy Chinese Academy of Medical Sciences Research Unit of Extracellular RNA State Key Laboratory of Pharmaceutical Biotechnology Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology NJU Advanced Institute of Life Sciences (NAILS) School of Life Sciences Nanjing University  Nanjing Jiangsu 210023 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,3]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pharmtox-010818-021041"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1249\/MSS.0000000000000975"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/387083a0"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI148372"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-14-0057"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2018.10.010"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12434"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-018-0983-6"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2023.03.036"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1830"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-021-00491-z"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awab354"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-023-06159-3"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2023.04.026"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4598(199904)22:4<460::AID-MUS6>3.0.CO;2-L"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddp395"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2010.151"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/gt.2009.59"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1807"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1065874"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd4467"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2023.03.028"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-023-01454-0"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.07.016"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra050541"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.122.001477"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00487.2002"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-0539"},{"key":"e_1_2_9_29_1","first-page":"233","volume":"1","author":"Meyer O. A.","year":"1979","journal-title":"Neurobehav. Toxicol."}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202502186","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.202502186","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202502186","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T11:41:15Z","timestamp":1769514075000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202502186"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,3]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1002\/adhm.202502186"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202502186","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,3]]},"assertion":[{"value":"2025-04-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02186"}}