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Replicating these biomechanical characteristics in engineered constructs remains a key challenge in regenerative medicine. This study presents a novel biofabrication platform for hybrid biopatches composed of tonsil\u2010derived mesenchymal stem cell (TMSC)\u2010laden collagen bioink reinforced with 3D printed polymeric patterns. Two distinct geometries, chiral and chevron, are designed to emulate the mechanical behavior of irregular and regular connective tissues, respectively. Mechanical testing shows that the chiral pattern exhibits quasi\u2010isotropic behavior with balanced stiffness and extensibility, whereas the chevron pattern demonstrate anisotropic mechanical properties. These mechanical features are maintained within the hybrid biopatches, leading to enhanced tensile strength and fatigue resistance compared with constructs composed solely of TMSC\u2010laden collagen. In a porcine mucosal defect model, the chiral\u2010patterned hybrid biopatch promoted superior epithelial repair, evidenced by narrower wound margins, continuous epithelial layers, and elevated expression of epithelial markers. These results suggest that mechanical compatibility with host tissue influences regenerative outcomes. Collectively, this study highlights the potential of incorporating geometric polymer patterns as a strategy for engineering tissue\u2010specific mechanics and improving regenerative performance, offering a promising platform for soft connective tissue repair.<\/jats:p>","DOI":"10.1002\/adhm.202502763","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T03:03:22Z","timestamp":1751857402000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["3D Bioprinting\u2010Assisted Engineering of Stem Cell\u2010Laden Hybrid Biopatches With Distinct Geometric Patterns Considering the Mechanical Characteristics of Regular and Irregular Connective Tissues"],"prefix":"10.1002","volume":"14","author":[{"given":"Minjun","family":"Ahn","sequence":"first","affiliation":[{"name":"Medical Research Institute Pusan National University  Yangsan 50612 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyu Tae","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Physiology Pusan National University School of Medicine  Yangsan 50612 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arvind Kumar","family":"Shukla","sequence":"additional","affiliation":[{"name":"School of Biomedical Convergence Engineering Pusan National University  Yangsan 50612 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boguen","family":"Kwon","sequence":"additional","affiliation":[{"name":"School of Biomedical Convergence Engineering Pusan National University  Yangsan 50612 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Ho","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Physiology Pusan National University School of Medicine  Yangsan 50612 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eui\u2010Suk","family":"Sung","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery, School of Medicine Pusan National University  Yangsan 50612 Republic of Korea"},{"name":"Research Institute for Convergence of Biomedical Science and Technology Pusan National University Yangsan Hospital  Yangsan 50612 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6693-0003","authenticated-orcid":false,"given":"Byoung Soo","family":"Kim","sequence":"additional","affiliation":[{"name":"Medical Research Institute Pusan National University  Yangsan 50612 Republic of Korea"},{"name":"School of Biomedical Convergence Engineering Pusan National University  Yangsan 50612 Republic of Korea"},{"name":"Research Institute for Convergence of Biomedical Science and Technology Pusan National University Yangsan Hospital  Yangsan 50612 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,7]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/0301-4622(88)87039-X"},{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2019.0243"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2009.07.040"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2016.04.006"},{"key":"e_1_2_9_3_2","first-page":"51","volume":"45","author":"Miller J. 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