{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T19:35:01Z","timestamp":1786476901073,"version":"build-2736575974"},"reference-count":63,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T00:00:00Z","timestamp":1767657600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T00:00:00Z","timestamp":1767657600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN\u20102025\u201005346"],"award-info":[{"award-number":["RGPIN\u20102025\u201005346"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Despite fast\u2010paced advances in bioengineering human\u2010based models, emulating fine\u2010tuned and interdependent immunoregulations of the human body remains challenging. While many bioengineered models contain immune cells, secondary lymphoid tissues are typically missing.<\/jats:p>\n                  <jats:p>\n                    Here, we report a self\u2010assembled, minimally functional lymphoreticular unit composed of fibroblastic reticular, lymphatic endothelial and CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells that emulate structural and functional interactions with the skin under inflammatory conditions. Following the optimization of the culture conditions, a layer\u2010by\u2010layer approach combining the relevant cell types yielded a self\u2010organized and compartmentalized lymphoreticular model mirroring human lymphoid tissue. Following verification of T cell motility, we established a skin\u2010lymphoreticular co\u2010culture within a microfluidic organ\u2010on\u2010chip to mimic interactions between skin and its draining lymph nodes. Co\u2010cultivation with atopic\u2010like skin models and the topical application of the skin sensitizer 2,4\u2010dinitrochlorobenzene stimulated strong migration and T cell infiltration from the lymphoreticular models while maintaining tissue integrity. The addition of anti\u2010inflammatory drugs abrogated the T cell mediated effects verifying the functionality of the model system. Taken together, the presented human\u2010based skin\u2010lymphoreticular model resembles a minimally functional unit that allows to study interactions between the skin and adjacent lymphoid tissues in a human\u2010based setup.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202503170","type":"journal-article","created":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T05:24:46Z","timestamp":1767763486000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A Human\u2010Based Skin\u2010Lymphoreticular Model\u2010on\u2010Chip to Emulate Inflammatory Skin Conditions"],"prefix":"10.1002","volume":"15","author":[{"given":"Zheng","family":"Tan","sequence":"first","affiliation":[{"name":"Faculty of Pharmaceutical Sciences The University of British Columbia  Vancouver BC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Partho 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