{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T01:58:51Z","timestamp":1785376731721,"version":"3.55.0"},"reference-count":66,"publisher":"Wiley","issue":"25","license":[{"start":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T00:00:00Z","timestamp":1771372800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T00:00:00Z","timestamp":1771372800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The unparalleled ability of aqueous two\u2010phase systems (ATPS) to reproduce microscale cellular and biomaterial compartmentalization to selectively modulate cell behavior and functionality is ideal for tissue engineering and regenerative medicine (TERM) purposes. Herein, we introduce new ATPS biomaterial inks for 3D bioprinting of water\u2010in\u2010water (W\/W) emulsions, enabling precise cellular crowding for tissue regeneration in vitro and ex vivo. Gelatin methacryloyl (GelMA) was dispersed in an alginic acid phase depending on sodium chloride (NaCl) concentration (0\u201036\u00a0g\/L). Emulsion droplet size (12.8\u00a0\u00b1\u00a02.6\u201352.4\u00a0\u00b1\u00a011.4\u00a0\u00b5m) influenced degradation and spatial cell localization (A549, C2C12, MG63). A microfluidic\u2010assisted 3D bioprinting approach allowed fine\u2010tuning of fiber structure adjusting ATPS deposition by modulating flow rates and printing speed. Rheological properties supported the findings of the two\u2010phase partitioning, aiding the selection of the ATPS ink formulation for functional cell\u2010laden construct fabrication. Encapsulation of C2C12 cells revealed enhanced cytoskeletal remodeling at higher salt concentrations. Increased GelMA phase promoted human bone marrow stromal cells (HBMSCs) crowding, mineral deposition and skeletal differentiation. In ovo studies demonstrated degradation control and vascular infiltration via salt modulation. Altogether, ATPS bioinks offer a versatile platform for the assembling of complex, hierarchical tissues with microscale precision, expanding biofabrication strategies for TERM applications.<\/jats:p>","DOI":"10.1002\/adhm.202503219","type":"journal-article","created":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T17:38:26Z","timestamp":1771436306000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Aqueous Two\u2010Phase Bioinks for Discrete Packing and Compartmentalization of 3D Bioprinted Cells"],"prefix":"10.1002","volume":"15","author":[{"given":"Martina","family":"Marcotulli","sequence":"first","affiliation":[{"name":"Center for Life Nano\u2010 &amp; Neuro\u2010 Science\u00a0\u2013 CLN2S Italian Institute of Technology (IIT)  Rome Italy"},{"name":"Department of Biochemical Sciences Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arianna","family":"Iacomino","sequence":"additional","affiliation":[{"name":"Center for Life Nano\u2010 &amp; Neuro\u2010 Science\u00a0\u2013 CLN2S Italian Institute of Technology (IIT)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Serpe","sequence":"additional","affiliation":[{"name":"Department of Chemistry Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Iafrate","sequence":"additional","affiliation":[{"name":"Center for Life Nano\u2010 &amp; Neuro\u2010 Science\u00a0\u2013 CLN2S Italian Institute of Technology (IIT)  Rome Italy"},{"name":"Department of Mechanical and Aerospace Engineering (DIMA) Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Bastioli","sequence":"additional","affiliation":[{"name":"Center for Life Nano\u2010 &amp; Neuro\u2010 Science\u00a0\u2013 CLN2S Italian Institute of Technology (IIT)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgia","family":"Montalbano","sequence":"additional","affiliation":[{"name":"Department of Applied Science and Technology Polytechnic University of Turin  Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"D'Orazio","sequence":"additional","affiliation":[{"name":"Department of Information Engineering Tor Vergata University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biagio","family":"Palmisano","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Franco","sequence":"additional","affiliation":[{"name":"Institute for Complex Systems National Research Council (ISC\u2010CNR)  Rome Italy"},{"name":"Physics Department Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arianna","family":"Mencattini","sequence":"additional","affiliation":[{"name":"Department of Information Engineering Tor Vergata University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Angelini","sequence":"additional","affiliation":[{"name":"Institute for Complex Systems National Research Council (ISC\u2010CNR)  Rome Italy"},{"name":"Physics Department Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenio","family":"Martinelli","sequence":"additional","affiliation":[{"name":"Department of Information Engineering Tor Vergata University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Corsi","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mara","family":"Riminucci","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giancarlo","family":"Ruocco","sequence":"additional","affiliation":[{"name":"Center for Life Nano\u2010 &amp; Neuro\u2010 Science\u00a0\u2013 CLN2S Italian Institute of Technology (IIT)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Scognamiglio","sequence":"additional","affiliation":[{"name":"Center for Life Nano\u2010 &amp; Neuro\u2010 Science\u00a0\u2013 CLN2S Italian Institute of Technology (IIT)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Barbetta","sequence":"additional","affiliation":[{"name":"Department of Chemistry Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9445-6994","authenticated-orcid":false,"given":"Gianluca","family":"Cidonio","sequence":"additional","affiliation":[{"name":"Center for Life Nano\u2010 &amp; Neuro\u2010 Science\u00a0\u2013 CLN2S Italian Institute of Technology (IIT)  Rome Italy"},{"name":"Department of Mechanical and Aerospace Engineering (DIMA) Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12575\u2010016\u20100048\u20108"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fluid.2019.112341"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201701036"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep11891"},{"issue":"95","key":"e_1_2_9_6_1","first-page":"1021","article-title":"Enhanced Production of Serratia marcescens Chitinase in PEG\/Dextran Aqueous Two\u2010phase Systems","volume":"0229","author":"ping C. J.","year":"1995","journal-title":"Enzyme and Microbial Technology"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.02.044"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0141\u20100229(94)90076\u20100"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jct.2012.07.017"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.3516658"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/c9cs00466a"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202204609"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/ar200294y"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201805460"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202106357"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40883\u2010022\u201000250\u20105"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202203243"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3MH01714A"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202400431"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758\u20105090\/ac5936"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1101\/2025.02.10.637484"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.4c22450"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/9783527838882.ch6"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201051958\u20104"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202214129"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232214366"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010015\u20106779\u20107"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering5030055"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2023.08.024"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10965\u2010019\u20101767\u20108"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.5772\/intechopen.75308"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2007.04.021"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymer.2020.122334"},{"key":"e_1_2_9_35_1","volume-title":"Interfacial Characterization of Immiscible Polymer Blends Using Rheology","author":"Sarathchandran C.","year":"2019"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s003970100189"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/jfb16010009"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1359\u20100294(03)00093\u20101"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22136758"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24065064"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1039\/d4ra08232j"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1385\/MB:19:3:269"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2014.10.035"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trechm.2022.10.009"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2014.00354"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1039\/c9bm01236b"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2012.0581"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2021.05.048"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1039\/c7bm00991g"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.11.169"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics16040469"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201024160\u20102"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2019.100028"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1039\/d1ra02744a"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2020.09.003"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2012.08.008"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758\u20105090\/ab8753"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSMBR.893727"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.129486"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/admt.202201244"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1002\/admt.202201102"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0138240"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201503310"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2016.12.016"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201004220\u20108"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1002\/9783527838882.ch6"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1080\/17452759.2024.2419411"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202503219","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.202503219","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202503219","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T01:25:44Z","timestamp":1785374744000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202503219"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,18]]},"references-count":66,"journal-issue":{"issue":"25","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/adhm.202503219"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202503219","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,18]]},"assertion":[{"value":"2025-06-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e03219"}}