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Kidney disease modeling in particular has progressed to using induced pluripotent stem cells (iPSCs) and microfluidic platforms to replicate the complex microenvironment of the kidney. However, current models lack mature tissue development, scalability, tunability, and spatial organization. In this study, a fully synthetic, 3D kidney disease platform that addresses these challenges is presented. This model comprises a compartmentalized poly (ethylene glycol) (PEG)\u2010based hydrogel matrix with anisotropic PEG\u2010based microgels. This multiphasic hydrogel system provides control over spatially organizing a triple\u2010co\u2010culture of key renal cell types: tubule\u2010epithelial cells (CD10\n                    <jats:sup>+<\/jats:sup>\n                    ), endothelial cells (CD31\n                    <jats:sup>+<\/jats:sup>\n                    ), and fibroblasts (PDGFR\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    ). Structural control and compartmentalization are enabled through enzymatically degradable rod microgels produced using microfluidics, allowing for a modular system. This study characterizes the synthetic models and analyzes the functionality of the system by examining cell\u2010material interactions. The use of this system as a promising disease model is demonstrated through the addition of TGF\u03b2, inducing fibrosis. This work highlights a novel approach to building a fully synthetic, scalable, modular kidney model with a tunable microenvironment.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202503287","type":"journal-article","created":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T05:41:06Z","timestamp":1761284466000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Development of a Synthetic 3D Platform for Compartmentalized Kidney In Vitro Disease Modeling"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1703-8554","authenticated-orcid":false,"given":"Ninon","family":"M\u00f6hl","sequence":"first","affiliation":[{"name":"Institute for Technical and Macromolecular Chemistry, RWTH Aachen University Chair for Macromolecular Materials for Medicine  52074 Aachen Germany"},{"name":"DWI\u2013Leibniz Institute for Interactive Materials  52074 Aachen Germany"},{"name":"Institute of 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