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A gelatin methacryloyl\u2013poly(ethylene glycol) diacrylate (GelMA\u2013PEGDA) resin was optimized for volumetric bioprinting. Compared to GelMA, Gel\u2013PEG enhanced printing fidelity, mechanical properties, and dimensional stability. Disc\u2010like constructs and channels with straight or angled geometries (60\u00b0, 90\u00b0, 110\u00b0) were fabricated and cultured with both cell types for up to 14 days. Label\u2010free holographic microscopy allowed real\u2010time visualization of cellular protrusions, critical for adhesion and mechanosensing, without staining, enabling long\u2010term live\u2010cell analysis in 3D constructs. HUVECs adhered, expressed CD31, and exhibited geometry\u2010dependent spreading, reflecting their native mechanosensitivity and alignment during vascular morphogenesis. In contrast, 143b cells spread uniformly, formed dense, geometry\u2010independent aggregates, and showed enhanced growth in Gel\u2013PEG compared to GelMA, consistent with their aggressive, metastatic behavior. These findings demonstrate that Gel\u2013PEG provides a stable, biomimetic matrix suitable for high\u2010resolution Vol3DP and that holographic microscopy enables dynamic assessment of cell\u2013material interactions. Together, they underscore the potential of this approach for engineering vascularized tissue models and for studying mechanobiological responses in both endothelial and cancer cell systems.<\/jats:p>","DOI":"10.1002\/adhm.202503550","type":"journal-article","created":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T07:40:57Z","timestamp":1762242057000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Geometrical Designs in Volumetric Bioprinting to Study Cellular Behaviors in Engineered Constructs"],"prefix":"10.1002","volume":"15","author":[{"given":"Julia","family":"Simi\u0144ska\u2010Stanny","sequence":"first","affiliation":[{"name":"Universit\u00e9 libre de Bruxelles (ULB) \u00c9cole Polytechnique de Bruxelles  3BIO\u2010BioMatter, Avenue F.D. 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