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Rapid pathogen identification is crucial, yet current workflows depend on multiple culture steps that delay pathogen identification and targeted treatment by days. A plug\u2010and\u2010play, fully automated centrifuge tube is presented that isolates and concentrates bacteria directly from blood or blood culture using only conventional lab centrifuges. Each tube can process 7.5 ml of sample and yields, within 40 min, a 0.7 mL clear suspension with greater than threefold enhanced bacteria concentration and  99.9% blood cell rejection, ready for downstream detection. It is demonstrated that this approach supports key diagnostic workflows, including 1) a novel isolate\u2010then\u2010culture strategy detecting bacterial concentrations as low as 10 CFU\/mL; 2) direct matrix\u2010assisted laser desorption ionization time\u2010of\u2010flight\u00a0(MALDI\u2010TOF) identification, bypassing subculturing, and; 3) microfluidic single\u2010cell detection. This fully automated platform is compatible with existing centrifuges, is anticipated to facilitate broader adoption in routine clinical practice, while its ability to enable rapid, same\u2010workshift bacterial enhancement can reduce diagnostic time by about one day in the context of time\u2010critical sepsis\u00a0diagnostics.<\/jats:p>","DOI":"10.1002\/adhm.202503651","type":"journal-article","created":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T10:56:06Z","timestamp":1756810566000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Plug\u2010and\u2010Play Centrifuge\u2010Only Device for Rapid Sepsis Diagnosis"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0477-6300","authenticated-orcid":false,"given":"Mohammad","family":"Osaid","sequence":"first","affiliation":[{"name":"Micro and Nanosystems KTH Royal Institute of Technology Stockholm 100 44 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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