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However, the suppressive tumor immune microenvironment usually inhibits the function of CTLs. Tumor\u2010associated macrophages (TAMs) play an important role in remodeling the tumor immune microenvironment. In this work, probiotic\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    Nissle 1917 (EcN) is used as a bioreactor to achieve intracellular synthesis of copper selenide nanoparticles (Cu\n                    <jats:sub>2\u2010x<\/jats:sub>\n                    Se NPs), giving Cu\n                    <jats:sub>2\u2010x<\/jats:sub>\n                    Se@EcN, which effectively repolarize macrophages from pro\u2010tumor M2 phenotype to anti\u2010tumor M1 phenotype mainly through activating MAPK signaling pathway. The synthesized Cu\n                    <jats:sub>2\u2010x<\/jats:sub>\n                    Se NPs exhibited excellent photothermal performance, inducing hyperthermia\u2010sensitized Fenton\u2010like reaction to catalyze the generation of hydroxyl radical from H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    , then resulted in immunogenic death effect of tumor cells and the repolarization of M2 macrophages. Combined with surface\u2010expressed pathogen\u2010associated molecular patterns (PAMPs) of bacteria, Cu\n                    <jats:sub>2\u2010x<\/jats:sub>\n                    Se@EcN synergistically promoted DCs maturation and TAMs reprogramming, eventually promoting the infiltration and activation of T cells. Therefore, the bacteria\u2010inorganic hybrid nanomaterials showed satisfied effect in inhibiting tumor growth and recurrence.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202503745","type":"journal-article","created":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T07:35:01Z","timestamp":1767598501000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Biosynthesis of Cu\n                    <sub>2\u2010x<\/sub>\n                    Se Nanocatalyst Within Probiotics for Repolarization of Tumor Associated Macrophages to Boost Mild Photothermal Sensitized Chemodynamic Immunotherapy"],"prefix":"10.1002","volume":"15","author":[{"given":"Meichan","family":"Wu","sequence":"first","affiliation":[{"name":"National Engineering Research Centre for Nanomedicine, College of Life Science and Technology Huazhong University of Science and Technology  Wuhan 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