{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T21:49:10Z","timestamp":1782510550346,"version":"3.54.5"},"reference-count":52,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T00:00:00Z","timestamp":1778803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T00:00:00Z","timestamp":1778803200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100010564","name":"Deutsches Zentrum f\u00fcr Lungenforschung","doi-asserted-by":"publisher","award":["82DZL00402"],"award-info":[{"award-number":["82DZL00402"]}],"id":[{"id":"10.13039\/501100010564","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003542","name":"Ministerium f\u00fcr Wissenschaft, Forschung und Kunst Baden-W\u00fcrttemberg","doi-asserted-by":"publisher","award":["7533\u20107\u201011.10\u20108"],"award-info":[{"award-number":["7533\u20107\u201011.10\u20108"]}],"id":[{"id":"10.13039\/501100003542","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Functional precision oncology aims to support personalized cancer therapy by assessing drug sensitivity in patient\u2010derived tumor cells ex vivo. However, conventional drug sensitivity and resistance testing (DSRT) platforms typically require large numbers of cells, limiting their applicability to surgically resected tumors and posing challenges for patients diagnosed at advanced stages, where only small biopsy samples are available. To address this limitation, we developed a miniaturized DSRT workflow based on a Droplet Microarray (DMA) chip, which comprises 672 hydrophilic spots separated by superhydrophobic borders and enables high\u2010throughput screening in nanoliter volumes. Using 300 cells per 200\u2010nL droplet, lung cancer cells freshly isolated from surgical specimens were tested against 12 compounds across five concentrations and five replicates (360 experimental conditions). This required approximately 120,000 cells total, including additional cells for handling and processing. The approach generated drug\u2010specific dose\u2013response profiles and variable IC\n                    <jats:sub>50<\/jats:sub>\n                    values across tumors of the same subtype. Comparable drug responses were also observed across three spatially distinct regions of the same tumor, indicating consistent assay performance. Overall, these results demonstrate that DSRT on the DMA platform is feasible with limited numbers of cells derived from clinical samples and may be useful for functional drug testing when tissue availability is constrained.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202503761","type":"journal-article","created":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T09:57:50Z","timestamp":1778839070000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Functional Precision Oncology Approach Using Nanoliter Droplet Array for Drug Sensitivity Testing in Lung Cancer"],"prefix":"10.1002","volume":"15","author":[{"given":"Maryam","family":"Salarian","sequence":"first","affiliation":[{"name":"Institute of Biological and Chemical Systems\u2013Functional Molecular Systems (IBCS\u2013FMS) Karlsruhe Institute of Technology (KIT)  Eggenstein\u2013Leopoldshafen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vicky L.","family":"Schiling","sequence":"additional","affiliation":[{"name":"Institute of Biological and Chemical Systems\u2013Functional Molecular Systems (IBCS\u2013FMS) Karlsruhe Institute of Technology (KIT)  Eggenstein\u2013Leopoldshafen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Muley","sequence":"additional","affiliation":[{"name":"Translational Research Unit (STF) Thoraxklinik at Heidelberg University Hospital  Heidelberg Germany"},{"name":"Translational Lung Research Center Heidelberg (TLRC) Member of the German Center for Lung Research (DZL)  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Allg\u00e4uer","sequence":"additional","affiliation":[{"name":"Institute of Pathology Heidelberg University Hospital  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Eichhorn","sequence":"additional","affiliation":[{"name":"Translational Lung Research Center Heidelberg (TLRC) Member of the German Center for Lung Research (DZL)  Heidelberg Germany"},{"name":"Department of Thoracic Surgery Thoraxklinik at Heidelberg University Hospital  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Meister","sequence":"additional","affiliation":[{"name":"Translational Research Unit (STF) Thoraxklinik at Heidelberg University Hospital  Heidelberg Germany"},{"name":"Translational Lung Research Center Heidelberg (TLRC) Member of the German Center for Lung Research (DZL)  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc A.","family":"Schneider","sequence":"additional","affiliation":[{"name":"Translational Research Unit (STF) Thoraxklinik at Heidelberg University Hospital  Heidelberg Germany"},{"name":"Translational Lung Research Center Heidelberg (TLRC) Member of the German Center for Lung Research (DZL)  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7054-0609","authenticated-orcid":false,"given":"Anna A.","family":"Popova","sequence":"additional","affiliation":[{"name":"Institute of Biological and Chemical Systems\u2013Functional Molecular Systems (IBCS\u2013FMS) Karlsruhe Institute of Technology (KIT)  Eggenstein\u2013Leopoldshafen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes11070747"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1142\/S2339547818300020"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276\u2010022\u201000864\u20103"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.19.03010"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010019\u20100407\u20105"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010019\u20100424\u20104"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2021.12.004"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/adtp.201900100"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159\u20108290.CD\u201021\u20100538"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/CM9.0000000000002529"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.4143\/crt.2022.264"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.21037\/tlcr\u201022\u2010148"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtho.2019.03.027"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.34739"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers16040778"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416\u2010020\u20100993\u20105"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201011867\u20106"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2022.04.006"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasc.2020.04.014"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420\u2010023\u201001722\u20105"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.5863\/1551-6776-27.2.123"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/2211068216677204"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201502115"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/2472630320934432"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/admi.201400269"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6RA06011K"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep05193"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15102709"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201031263\u2010x"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.11.051"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202300591"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/adbi.202200166"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1111"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010024\u201002848\u20104"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.2165\/00002512\u2010199405030\u201000006"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.3816\/CLC.2004.s.006"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/10.suppl_5.S63"},{"key":"e_1_2_8_39_1","first-page":"209","article-title":"Gemcitabine for the Treatment of Advanced Nonsmall Cell Lung Cancer","volume":"2","author":"Toschi L.","year":"2009","journal-title":"OncoTargets and Therapy"},{"key":"e_1_2_8_40_1","first-page":"63","article-title":"Docetaxel in the Treatment of Non\u2010Small Cell Lung Carcinoma: An Update and Analysis","volume":"1","author":"Gubens M. A.","year":"2010","journal-title":"Lung Cancer: Targets and Therapy"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26020796"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2016.01.003"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1159\/000227259"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935\u2010022\u201002624\u20109"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495\u2010199958030\u201000020"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2021.119527"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495\u2010199958003\u201000002"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2020.107753"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a026781"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300060520955058"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2023.1089681"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1986.192"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010018\u20100096\u20105"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202503761","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.202503761","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202503761","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T21:28:20Z","timestamp":1782509300000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202503761"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,15]]},"references-count":52,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/adhm.202503761"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202503761","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,15]]},"assertion":[{"value":"2025-08-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e03761"}}