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This study identified that CrfP, a murein hydrolase that induces BG production in\n                    <jats:italic>S. suis<\/jats:italic>\n                    (SSBGs), functions by forming pores during natural transformation. A shuttle plasmid pKSET CrfP is then constructed to achieve anhydrotetracycline\u2010inducible SSBGs generation. The screened WR\n                    <jats:sub>12<\/jats:sub>\n                    , a cell\u2010penetrating peptide (CPP), significantly enhanced the production of SSBGs and achieved a 100% inactivation for vaccine preparation. Immunization with SSBGs elicited a robust immune response, characterized by the production of IgG1 and IgG2a antibodies and the activation of CD4\n                    <jats:sup>+<\/jats:sup>\n                    T lymphocytes, crucial for humoral immunity. The SSBGs vaccine exhibited excellent protective efficacy against\n                    <jats:italic>S. suis<\/jats:italic>\n                    challenge in a mouse model. These findings establish a scalable method for Gram\u2010positive BG vaccines and introduce CrfP as a template for lytic enzyme discovery in other pathogens. The work advances a versatile platform for next\u2010generation vaccines against emerging infections.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202503790","type":"journal-article","created":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T06:57:39Z","timestamp":1764658659000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Engineered\n                    <i>Streptococcus suis<\/i>\n                    Bacterial Ghosts via Lytic Plasmid and Cell\u2010Penetrating Peptides for Enhanced Protective Immunity"],"prefix":"10.1002","volume":"15","author":[{"given":"Jianan","family":"Liu","sequence":"first","affiliation":[{"name":"MOE Joint International Research Laboratory of Animal Health and Food Safety College of Veterinary Medicine Nanjing Agricultural University  Nanjing China"},{"name":"WOAH Reference Lab for Swine Streptococcosis  Nanjing 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