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The stromal vascular fraction (SVF) is a heterogenous cell isolate, that may improve nerve regeneration outcomes when transplanted at the site of a nerve defect. Self\u2010Assembling Peptide hydrogels (SAPH) are synthetic materials derived from short chains of biological amino acids. They are safe, injectable hydrogels whose charge and mechanical properties are easily tuned. Our hypothesis is that SVF, when transplanted within an SAPH, tailored toward nerve regeneration and SVF viability, will improve outcomes of nerve regeneration. In vitro modelling is used to select SAPH that support the viable 3D culture of SVF and outgrowth from neuronal explants. In vivo experimentation with a 10\u00a0mm rat sciatic nerve defect demonstrated that SVF, when delivered within a conduit, in a positively charged, mechanically optimized SAPH, significantly improved functional motor and sensory recovery compared to collagen controls and SAPH without SVF and is at least as good as autograft. Using male SVF, qPCR identification of Y chromosomal DNA suggested that SVF transplanted in SAPH has increased longevity when compared to SVF transplanted in collagen gel.<\/jats:p>","DOI":"10.1002\/adhm.202503987","type":"journal-article","created":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T06:47:56Z","timestamp":1765349276000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Self\u2010Assembling Peptide Hydrogels Support Stromal Vascular Fraction Viability to Promote In Vivo Nerve Regeneration"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9354-372X","authenticated-orcid":false,"given":"Liam A.","family":"McMorrow","sequence":"first","affiliation":[{"name":"Blond McIndoe Laboratories Division of Cell Matrix Biology and Regenerative Medicine School of Biological Sciences Faculty of Biology Medicine and Health Manchester Academic Health Science Centre University of Manchester  Manchester UK"},{"name":"Department of Plastic Surgery &amp; 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