{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T05:32:34Z","timestamp":1788845554554,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T00:00:00Z","timestamp":1770854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T00:00:00Z","timestamp":1770854400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Duchenne muscular dystrophy (DMD) is a genetic disorder characterized by progressive muscle degeneration that significantly reduces the quality of life and lifespan of patients. Currently, cardiomyopathy represents the leading cause of death in later stages of the disease. While calcium dysregulation, fibrosis, and fat deposits are well\u2010documented hallmarks of DMD cardiomyopathies, the exact pathogenic mechanisms remain unclear due to the lack of reliable models. In this study, we developed 3D cardiac organoids (COs) from DMD patient\u2010derived induced pluripotent stem cells (DMD\u2010hiPSCs), their isogenic control (DMD\u2010Iso\u2010hiPSCs), and a healthy hiPSC line (HC\u2010hiPSCs). By day 15 of cardiac differentiation, DMD\u2010COs exhibited key disease features, including increased cell death, elevated ROS levels, and calcium signaling defects, compared with controls. Leveraging bioprinting technology, COs were embedded in a 7% alginate\u20135% gelatin hydrogel to generate bioprinted constructs (HC\u2010bCOs, DMD\u2010Iso\u2010bCOs, and DMD\u2010bCOs). These constructs self\u2010organized, displaying increased cell\u2013cell communication, and reduced levels of the early cardiac transcription factor NKX2.5. By day 14 post\u2010bioprinting, DMD\u2010bCOs showed increased cell death and dysregulated expression of cardiac and fibrotic markers, mimicking DMD\u2010associated cardiomyopathy. This study demonstrates the potential of both COs and bCOs as a tool for studying DMD cardiomyopathy and advancing drug screening and therapies.<\/jats:p>","DOI":"10.1002\/adhm.202504004","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T12:38:18Z","timestamp":1770899898000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Patient\u2010Derived 3D Bioprinted Cardiac Organoid Constructs Reveal Key Pathological Features of Duchenne Muscular Dystrophy"],"prefix":"10.1002","volume":"15","author":[{"given":"Vittoria","family":"Marini","sequence":"first","affiliation":[{"name":"Translational Cardiology Laboratory Stem Cell &amp; Developmental Biology Unit Department of Development &amp; Regeneration KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margalida","family":"Campaner Socias","sequence":"additional","affiliation":[{"name":"Translational Cardiology Laboratory Stem Cell &amp; Developmental Biology Unit Department of Development &amp; Regeneration KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Dimopoulos","sequence":"additional","affiliation":[{"name":"Skeletal Biology and Engineering Research Centre Department of Development &amp; Regeneration KU Leuven  Leuven Belgium"},{"name":"Prometheus \u2010 the Leuven R&amp;D Translational Division of Skeletal Tissue Engineering KU Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenza","family":"Rinvenuto","sequence":"additional","affiliation":[{"name":"Translational Cardiology Laboratory Stem Cell &amp; Developmental Biology Unit Department of Development &amp; Regeneration KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico","family":"Pozzo","sequence":"additional","affiliation":[{"name":"Department of Radiotherapy and Radiosurgery IRCCS Humanitas Research Hospital  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Stalkopf","sequence":"additional","affiliation":[{"name":"Translational Cardiology Laboratory Stem Cell &amp; Developmental Biology Unit Department of Development &amp; Regeneration KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo","family":"Serafini","sequence":"additional","affiliation":[{"name":"Translational Cardiology Laboratory Stem Cell &amp; Developmental Biology Unit Department of Development &amp; Regeneration KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Morri","sequence":"additional","affiliation":[{"name":"Translational Cardiology Laboratory Stem Cell &amp; Developmental Biology Unit Department of Development &amp; Regeneration KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashley","family":"Wang","sequence":"additional","affiliation":[{"name":"Translational Cardiology Laboratory Stem Cell &amp; Developmental Biology Unit Department of Development &amp; Regeneration KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"La Rovere","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular &amp; Cellular Signaling Department of Cellular &amp; Molecular Medicine KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoke Chin","family":"Chai","sequence":"additional","affiliation":[{"name":"Interuniversity Microelectronics Center (imec)  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sveva","family":"Bollini","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine (DIMES) University of Genova  Genova Italy"},{"name":"IRCCS Ospedale Policlinico San Martino  Genova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geert","family":"Bultynck","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular &amp; Cellular Signaling Department of Cellular &amp; Molecular Medicine KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. Llewelyn","family":"Roderick","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Cardiology Department of Cardiovascular Sciences KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis","family":"Papantoniou","sequence":"additional","affiliation":[{"name":"Skeletal Biology and Engineering Research Centre Department of Development &amp; Regeneration KU Leuven  Leuven Belgium"},{"name":"Prometheus \u2010 the Leuven R&amp;D Translational Division of Skeletal Tissue Engineering KU Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2422-3757","authenticated-orcid":false,"given":"Maurilio","family":"Sampaolesi","sequence":"additional","affiliation":[{"name":"Translational Cardiology Laboratory Stem Cell &amp; Developmental Biology Unit Department of Development &amp; Regeneration KU Leuven  Leuven Belgium"},{"name":"Histology and Medical Embryology Unit Department of Anatomy, Histology, Forensic Medicine and Orthopedics Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572\u2010021\u201000248\u20103"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ncl.2014.05.002"},{"key":"e_1_2_10_4_1","first-page":"1361","article-title":"Duchenne Muscular Dystrophy","volume":"134","author":"Annexstad E. J.","year":"2014","journal-title":"Duchenne Muscular Dystrophy"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics15030778"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpc.12868"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.878311"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9020520"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00007.2015"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2016.02.081"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.5772\/65061"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2025.07.031"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.018424"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13395\u2010024\u201000353\u20103"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2021.12.019"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13311\u2010022\u201001197\u20109"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.18063\/ijb.v7i3.364"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.61621"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.2217\/3dp\u20102018\u20100017"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2024.115237"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2021.03.016"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2958"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44222\u2010024\u201000268\u20100"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43586\u2010021\u201000073\u20108"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015\u2010022\u201010385\u20101"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204\u2010021\u201003212\u2010y"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.019505"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010021\u201003862\u20102"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2024.103319"},{"key":"e_1_2_10_30_1","doi-asserted-by":"crossref","unstructured":"S.Sun T.Yu J. Y.Huh Y.Cai S.Yoon andH. M. A.Javaid Aminoguanidine Hemisulfate Improves Mitochondrial Autophagy Oxidative Stress and Muscle Force in Duchenne Muscular Dystrophy via the AKT\/FOXO1 Pathway in Mdx Mice. Skelet Muscle 15 no.1(2025):2 https:\/\/doi.org\/10.1186\/s13395\u2010024\u201000371\u20101.","DOI":"10.1186\/s13395-024-00371-1"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2021.04.018"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.1042030"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2019.02.049"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201091575\u20108"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a035428"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvz109"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.647010"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00890.2013"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202206264"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201005018\u20104"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2021.636257"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202302229"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aaw2459"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201022225\u2010w"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202404251"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201021029\u20102"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758\u20105090\/ac47d8"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1161\/RES.0000000000000110"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202504004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.202504004","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202504004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T09:08:53Z","timestamp":1781514533000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202504004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,12]]},"references-count":48,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/adhm.202504004"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202504004","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,12]]},"assertion":[{"value":"2025-08-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e04004"}}