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Osteoarthritis (OA), affecting over 590 million people, is a prevalent fibrotic joint disease for which disease\u2010modifying therapies remain lacking, partly because disease progression remains incompletely understood. As human\u2010relevant in vitro models, including organ\u2010on\u2010chip and microphysiological systems, become increasingly important, non\u2010invasive technologies capable of continuous cellular monitoring are urgently needed. Here, we implement high\u2010density complementary metal\u2010oxide\u2010semiconductor (CMOS) multielectrode array (MEA)\u2010based impedance profiling as a label\u2010free platform for longitudinal assessment of OA\u2010relevant chondrocyte remodeling. Human primary chondrocytes cultured on CMOS\u2010MEA chips exhibited impedance increases that correlated with electrode occupancy and extracellular matrix (ECM) deposition. Spatial impedance mapping enabled visualization of injury\u2010response dynamics, while oxidative stress induced progressive impedance decline accompanied by apoptosis, morphological remodeling, and chondrocyte dedifferentiation, evidenced by reduced collagen II and SOX9 expression and increased collagen I expression. In contrast, osteogenic de\u2010differentiation produced increased impedance associated with ECM remodeling and matrix mineralization. Together, these findings support high\u2010density CMOS\u2010MEA impedance profiling as a non\u2010invasive approach for monitoring cellular remodeling and provide a foundation for future integration of spatially resolved impedance sensing into advanced microphysiological systems for OA and other fibrotic diseases.<\/jats:p>","DOI":"10.1002\/adhm.202504115","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T10:52:47Z","timestamp":1791975167000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single\u2010Cell Resolution Mapping of Osteoarthritis\u2010Associated Chondrocyte Dynamics Using a High\u2010Density CMOS MEA Platform"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3740-2324","authenticated-orcid":false,"given":"Neha","family":"Deshpande","sequence":"first","affiliation":[{"name":"imec  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6761-3671","authenticated-orcid":false,"given":"Pauline","family":"Coquart","sequence":"additional","affiliation":[{"name":"imec  Leuven Belgium"},{"name":"Department of Physics and Astronomy KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bram","family":"Theuns","sequence":"additional","affiliation":[{"name":"imec  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Cremery","sequence":"additional","affiliation":[{"name":"imec  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime Rodolfo V. 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