{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T20:27:30Z","timestamp":1788380850966,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","issue":"25","license":[{"start":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T00:00:00Z","timestamp":1780704000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T00:00:00Z","timestamp":1780704000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82470988"],"award-info":[{"award-number":["82470988"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007957","name":"Chongqing Municipal Education Commission","doi-asserted-by":"publisher","award":["KJZD\u2010K202500412"],"award-info":[{"award-number":["KJZD\u2010K202500412"]}],"id":[{"id":"10.13039\/501100007957","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100019531","name":"Science Fund for Distinguished Young Scholars of Chongqing Municipality","doi-asserted-by":"publisher","award":["CSTB2023NSCQ\u2010JQX0006"],"award-info":[{"award-number":["CSTB2023NSCQ\u2010JQX0006"]}],"id":[{"id":"10.13039\/501100019531","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    There is an urgent need to overcome the limitations associated with limited autologous bone availability, allogeneic bone rejection, and the insufficient bioactivity of traditional materials for bone\u2010defect repair. In this study, skin secretions from\n                    <jats:italic>Andrias davidianus<\/jats:italic>\n                    (SSAD) were compounded with methacrylated gelatin (GelMA) to develop a novel bioink and construct bone\u2010regeneration scaffolds loaded with osteoblasts via extrusion\u2010based 3D bioprinting. The optimized GelMA\/SSAD bioink (50% v\/v SSAD, 6% w\/v GelMA) demonstrated excellent printability characterized by shear\u2010thinning behavior and high\u2010resolution structure formation and significantly enhanced mechanical strength. In vitro studies demonstrated that the scaffolds were not only biocompatible but also effective in promoting osteoblast proliferation, differentiation, and mineralization, as evidenced by significantly increased ALP activity and the upregulated expression of key osteogenic genes (RUNX2 and OCN). In a rat cranial bone defect model, the new bone volume fraction of the GelMA\/SSAD scaffolds (12.54%) was markedly higher than that of the pure GelMA control group (9.82%) eight weeks after implantation. Multimodal analyses revealed that the scaffold synergistically accelerated bone regeneration by promoting TRAP\u2010positive osteoclast activity and increasing type III collagen deposition. Overall, this SSAD\u2010based bioink combines excellent printability, biocompatibility, and strong osteogenic activity, thereby providing a promising strategy for functional bone repair.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202504181","type":"journal-article","created":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T04:36:07Z","timestamp":1780806967000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["3D Bioprinting of Giant Salamander Mucin\u2010Based Bioink for Enhanced Bone Regeneration"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5420-6870","authenticated-orcid":false,"given":"Yucan","family":"Jiang","sequence":"first","affiliation":[{"name":"The Affiliated Stomatological Hospital of Chongqing Medical University  Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Diseases  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering Of Higher Education  Chongqing China"},{"name":"Chongqing Municipal Health Commission Key Laboratory of Oral Biomedical Engineering  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Zhu","sequence":"additional","affiliation":[{"name":"The Affiliated Stomatological Hospital of Chongqing Medical University  Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Diseases  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering Of Higher Education  Chongqing China"},{"name":"Chongqing Municipal Health Commission Key Laboratory of Oral Biomedical Engineering  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruyi","family":"Dang","sequence":"additional","affiliation":[{"name":"The Affiliated Stomatological Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Li","sequence":"additional","affiliation":[{"name":"The Affiliated Stomatological Hospital of Chongqing Medical University  Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Diseases  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering Of Higher Education  Chongqing China"},{"name":"Chongqing Municipal Health Commission Key Laboratory of Oral Biomedical Engineering  Chongqing China"},{"name":"The Maternal and Child Health Care Hospital of Xiangtan City  Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangfan","family":"Xiang","sequence":"additional","affiliation":[{"name":"The Affiliated Stomatological Hospital of Chongqing Medical University  Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Diseases  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering Of Higher Education  Chongqing China"},{"name":"Chongqing Municipal Health Commission Key Laboratory of Oral Biomedical Engineering  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shizhu County People's Hospital  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufei","family":"Ma","sequence":"additional","affiliation":[{"name":"Research Center of Stem Cells and Ageing Chongqing Institute of Green and Intelligent Technology Chinese Academy of Sciences  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4070-1958","authenticated-orcid":false,"given":"Ximu","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Affiliated Stomatological Hospital of Chongqing Medical University  Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Diseases  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering Of Higher Education  Chongqing China"},{"name":"Chongqing Municipal Health Commission Key Laboratory of Oral Biomedical Engineering  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202302818"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmpb.2022.106958"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.81480"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967\u2010024\u201005809\u20100"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202101452"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741\u20107015\u20109\u201066"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2017.05.007"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201048023\u20108"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202403641"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2022.100382"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010022\u201032868\u2010y"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2022.100237"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.08.045"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2024.150884"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2024.04.032"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2021.03.010"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2021.11.030"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202501254"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.103725"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40824\u2010023\u201000422\u20106"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201809110"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202309774"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202105255"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compositesb.2024.111261"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202315590"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3bm00012e"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1088\/1361\u2010665X\/ac976a"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.TEB.2012.0437"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.0c06083"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.07.164"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202414362"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2023.121567"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym15081915"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2025.01.033"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202106824"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202417801"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.1053491"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.08.005"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.3c03659"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2025.123253"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010physiol\u2010021119\u2010034425"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2008.03.027"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11154\u2010010\u20109153\u20101"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.26502\/fccm.92920302"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223\u2010014\u20109843\u2010x"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1177\/1358863\u00d711402249"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1088\/2631\u20107990\/acfcf2"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.26599\/OSHM.2025.9610031"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202504181","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.202504181","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202504181","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T01:26:36Z","timestamp":1785374796000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202504181"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,6]]},"references-count":48,"journal-issue":{"issue":"25","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/adhm.202504181"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202504181","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,6]]},"assertion":[{"value":"2025-08-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e04181"}}