{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T15:49:05Z","timestamp":1782402545996,"version":"3.54.5"},"reference-count":74,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T00:00:00Z","timestamp":1778025600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T00:00:00Z","timestamp":1778025600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100012818","name":"Comunidad de Madrid","doi-asserted-by":"publisher","award":["P022\/BMD\u20107236"],"award-info":[{"award-number":["P022\/BMD\u20107236"]}],"id":[{"id":"10.13039\/100012818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003852","name":"Regione Campania","doi-asserted-by":"publisher","award":["L.R. N.5 Bando 2003"],"award-info":[{"award-number":["L.R. N.5 Bando 2003"]}],"id":[{"id":"10.13039\/501100003852","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>There is broad consensus that successful repair of severe peripheral nerve injuries requires recreating key structural and cellular features of the natural regenerative process, particularly the action of Bands of B\u00fcngner (BoB), longitudinal Schwann cell (SC) structures that guide regenerating axons. Current biomaterial\u2010based strategies have shown limited efficacy, in part because they do not sufficiently reproduce the anisotropic and cellular microenvironment established by BoB, resulting in disorganized axonal growth and reduced regenerative efficiency across long gaps. To address this limitation, a biohybrid scaffold designed to promote Schwann cell self\u2010organization into B\u00fcngner\u2010like structures through defined physical cues. Rather than relying solely on biochemical supplementation is developed, this system leverages anisotropic fiber architecture to induce SC alignment and early activation\u2010associated phenotypic modulation. In this study, a self\u2010organizing biohybrid BoB (BBoB) construct formed by Schwann cells within an aligned fiber\u2010based scaffold is presented. It is demonstrated that these engineered structures recapitulate key morphological features of native BoB in vitro and promote enhanced axonal regeneration across a 11\u00a0mm sciatic nerve defect in vivo. Together, these findings support the concept that physically programmed Schwann cell organization within biomaterial conduits can enhance peripheral nerve regeneration, using clinically accessible biomaterials and autologous Schwann cells.<\/jats:p>","DOI":"10.1002\/adhm.202504185","type":"journal-article","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T08:56:56Z","timestamp":1778057816000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Self\u2010Assembling Biohybrid Bands of B\u00fcngner for Axonal Regeneration in the Peripheral Nervous System"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0544-9574","authenticated-orcid":false,"given":"Atocha","family":"Guedan\u2010Duran","sequence":"first","affiliation":[{"name":"Neuro\u2010computing &amp; Neuro\u2010robotics Research Group Complutense University of Madrid  Madrid Spain"},{"name":"Bioactive Surfaces SL  Madrid Spain"},{"name":"Silk Biomed SL  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3518-0262","authenticated-orcid":false,"given":"Nahla","family":"Jemni\u2010Damer","sequence":"additional","affiliation":[{"name":"Neuro\u2010computing &amp; Neuro\u2010robotics Research Group Complutense University of Madrid  Madrid Spain"},{"name":"Bioactive Surfaces SL  Madrid Spain"},{"name":"Silk Biomed SL  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasmine","family":"Owen","sequence":"additional","affiliation":[{"name":"Neuro\u2010computing &amp; Neuro\u2010robotics Research Group Complutense University of Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Murciano","sequence":"additional","affiliation":[{"name":"Faculty of Biology Complutense University of Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria\u2010Dolores Gimeno","family":"Garc\u00eda\u2010Andrade","sequence":"additional","affiliation":[{"name":"Hand and Wrist Surgery Unit, Orthopedic Surgery and Traumatology Department San Carlos Clinical Hospital  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Assunta","family":"Virtuoso","sequence":"additional","affiliation":[{"name":"Department of Mental and Physical Health and Preventive Medicine Division of Human Anatomy, Laboratory of Morphology of Neuronal Networks &amp; Systems Biology University of Campania \u201cLuigi Vanvitelli\u201d  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Cirillo","sequence":"additional","affiliation":[{"name":"Department of Mental and Physical Health and Preventive Medicine Division of Human Anatomy, Laboratory of Morphology of Neuronal Networks &amp; Systems Biology University of Campania \u201cLuigi Vanvitelli\u201d  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Papa","sequence":"additional","affiliation":[{"name":"Department of Mental and Physical Health and Preventive Medicine Division of Human Anatomy, Laboratory of Morphology of Neuronal Networks &amp; Systems Biology University of Campania \u201cLuigi Vanvitelli\u201d  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0897-411X","authenticated-orcid":false,"given":"Fivos","family":"Panetsos","sequence":"additional","affiliation":[{"name":"Neuro\u2010computing &amp; Neuro\u2010robotics Research Group Complutense University of Madrid  Madrid Spain"},{"name":"Bioactive Surfaces SL  Madrid Spain"},{"name":"Silk Biomed SL  Madrid Spain"},{"name":"Neural Plasticity Research Group Institute For Health Research San Carlos Clinical Hospital (IdISSC)  Madrid Spain"},{"name":"Omnia Mater SL  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2020.584823"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222413380"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.151704"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/1073858415572361"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22852"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100913-013101"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a020529"},{"key":"e_1_2_11_9_1","first-page":"369","article-title":"Experiments on the Section of the Glosso\u2010Pharyngeal and Hypoglossal Nerves of the Frog, and Observations of the Alterations Produced Thereby in the Structure of Their Primitive Fibres","volume":"76","author":"Waller A.","year":"1851","journal-title":"Edinburgh Medical and Surgical Journal"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1529-8027.2002.02002.x"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-015-1482-4"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2012.08.008"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18020287"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2014.11.010"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.L.00704"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3171\/FOC.2009.26.2.E3"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0094-1298(02)00101-3"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1447-073x.2004.00090.x"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1620-06.2006"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2005.12.018"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0612-07.2007"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2011.12.025"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/698256"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2014.10.008"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2014.03.018"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP270874"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/1558944716643277"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.10945"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2018.05.016"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2018.09.020"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.2002.01891.x"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02740621"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.5435\/00124635-200007000-00005"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-198610000-00040"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/4969523"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0022-7722.2002.00012.x"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1080\/02844310410017979"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.3171\/FOC.2009.26.2.E4"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0110090"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.253511"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2020.580654"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.07.007"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2022.948454"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/jfb14050238"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2015.10.040"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2015.08.010"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cnp.2024.04.002"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2010.09.011"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.03168"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2015.04.003"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano13182528"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S436871"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12951-024-02475-8"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-15639-z"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21207467"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41536-025-00443-w"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.5c01600"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1764-14.2014"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.00735"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/8124444"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1039\/B9NR00243J"},{"key":"e_1_2_11_62_1","article-title":"Bioengineered Nerve Conduits and Wraps for Peripheral Nerve Repair of the Upper Limb","volume":"12","author":"Thomson S. E.","year":"2022","journal-title":"Cochrane Database of Systematic Reviews"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.06.021"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(00)00278-7"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2752(1998)18:7<397::AID-MICR2>3.0.CO;2-F"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms160612925"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2020.580654"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.215266"},{"key":"e_1_2_11_69_1","doi-asserted-by":"publisher","DOI":"10.3171\/2020.8.JNS202349"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202004555"},{"key":"e_1_2_11_71_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-018-0491-8"},{"key":"e_1_2_11_72_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9092113"},{"key":"e_1_2_11_73_1","doi-asserted-by":"publisher","DOI":"10.5966\/sctm.2013-0011"},{"key":"e_1_2_11_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2018.07.048"},{"key":"e_1_2_11_75_1","first-page":"392","article-title":"Remyelination Improvement After Neurotrophic Factors Secreting Cells Transplantation in Rat Spinal Cord Injury","volume":"20","author":"Razavi S.","year":"2017","journal-title":"Iranian Journal of Basic Medical Sciences"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202504185","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.202504185","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202504185","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T15:22:49Z","timestamp":1782400969000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202504185"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,6]]},"references-count":74,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/adhm.202504185"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202504185","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,6]]},"assertion":[{"value":"2025-08-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e04185"}}