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Herein, a new Ad5\u2010FMD vaccine stabilizing and delivery technology is presented, termed as Stabilized Prophylactic Adenovirus Nanostructure Delivery via Microneedles (SPAND\u2010MN), which enables room\u2010temperature storage and easy\u2010to\u2010use, painless transdermal administration of Ad vectored vaccines. It is demonstrated the success of SPAND\u2010MN platform to deliver replication\u2010defective Adenovirus serotype 5 (Ad5) vaccine vectors expressing various antigens, including Green Fluorescent Protein (GFP), Luciferase (Luc), and Foot\u2010and\u2010Mouth Disease (FMD) capsid proteins. The SPAND\u2010MN formulation preserved transduction capacity of the Ad5 vaccines after exposure to an accelerated temperature (at 60\u00b0C) and a long\u2010term storage at 45\u00b0C for 1 month. In vitro and in vivo studies confirmed effective transduction efficiency, dose delivery, and biocompatibility of the SPAND\u2010MN platform. In vivo safety assessments revealed no cytotoxicity, systemic inflammation, and organ toxicity. SPAND\u2010MNs maintained immunogenicity equivalent to injections of the stock refrigerated vaccines even after thermal challenges. These results demonstrate the potential of SPAND\u2010MNs to offer a powerful, easy\u2010to\u2010use, cold\u2010chain free, and painless transdermal skin patch for Ad5 vaccination.<\/jats:p>","DOI":"10.1002\/adhm.202504342","type":"journal-article","created":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T09:15:11Z","timestamp":1776244511000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Microneedles Based Thermally Stable Ad\u20105 Viral Vector for Vaccine Delivery"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7858-8973","authenticated-orcid":false,"given":"Parbeen","family":"Singh","sequence":"first","affiliation":[{"name":"Mechanical Engineering Department University of Connecticut  Storrs Connecticut USA"},{"name":"Department of Biomedical Engineering University of Connecticut  Storrs Connecticut 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