{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T21:42:16Z","timestamp":1792186936789,"version":"4.4.0"},"reference-count":41,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81903201"],"award-info":[{"award-number":["81903201"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2022LSW011"],"award-info":[{"award-number":["ZR2022LSW011"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Vitiligo, an autoimmune skin disorder, is characterized by the loss of melanocytes, resulting in significant skin depigmentation. Ruxolitinib (RUX), which could target the JAK\u2010STAT signaling pathway, plays a crucial role in vitiligo treatment but faces great challenges in transdermal delivery. Here, we developed RUX\u2010laden silk fibroin nanoparticles to achieve transdermal delivery with the assistance of a CO\n                    <jats:sub>2<\/jats:sub>\n                    ablative laser. In vitro assays demonstrated that the RUX\u2010laden silk nanoparticles significantly inhibited the secretion of CXCL9 and CXCL10, and the phosphorylation of AKT2 and STAT3, indicating effective modulation of the JAK\u2010STAT pathway. The RUX\u2010laden silk nanoparticles exhibited excellent transdermal delivery capacity comparable to the Opzelura cream, which was further improved through a laser\u2010assisted strategy. In vivo studies revealed that the transdermally delivered RUX\u2010laden nanoparticles exhibited better skin toleration and significantly reduced the expression of multiple inflammatory cytokines such as CXCL9, CXCL10, IFN\u2010\u03b3 and TNF\u2010\u03b1, bringing substantial melanocyte recovery. The findings underscore the potential of silk\u2010based delivery systems in enhancing therapeutic efficacy against vitiligo, which would improve pigmentation restoration and inflammation modulation in skin disorders.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202504458","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T07:44:18Z","timestamp":1777016658000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transdermal Delivery of Ruxolitinib Through CO\n                    <sub>2<\/sub>\n                    Ablative Laser \u2010Assisted Silk Nanovehicle System for Improved Melanocyte Recovery in Vitiligo"],"prefix":"10.1002","volume":"15","author":[{"given":"Xue","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Dermatology Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qirui","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology Zhongshan Hospital Affiliated to Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawei","family":"Song","sequence":"additional","affiliation":[{"name":"Taian City Central Hospital  Tai An China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liying","family":"Xiao","sequence":"additional","affiliation":[{"name":"Institutes for Translational Medicine Soochow University  Suzhou China"},{"name":"National Engineering Laboratory for Modern Silk &amp; Collaborative Innovation Center of Suzhou Nano Science and Technology Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"Department of Laser and Aesthetic Medicine Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4889-5299","authenticated-orcid":false,"given":"Qiang","family":"Lu","sequence":"additional","affiliation":[{"name":"Institutes for Translational Medicine Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/1346-8138.15743"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.986918"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms242417306"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2118828"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389200218666170222150555"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.24875\/PJDV.22000037"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.19162"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.202300141"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics15020355"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.22606"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/9781839162664-00064"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2015.10.049"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.1c01122"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2021.804415"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnsc.2020.09.016"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-021-03904-9"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/jocd.16514"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22137107"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.2c00666"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.9b00621"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm500662z"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2014.05.059"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8TB00607E"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.7b00969"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jddst.2022.103997"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/masy.202400041"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/12034754251320637"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1080\/10717544.2021.1958106"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-97296-2"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm13175225"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3007811"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2018.12.012"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2021.121242"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7CP03258G"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-024-02027-2"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1177\/10600280221143748"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amsu.2022.104499"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/14740338.2024.2446432"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2022.09.025"},{"key":"e_1_2_9_41_1","volume-title":"Innovative Transdermal Drug Delivery Systems: Benefits, Challenges, and Emerging Applications","author":"Shah S. W. A.","year":"2025"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/pat.6406"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202504458","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.202504458","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202504458","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T21:19:09Z","timestamp":1792185549000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202504458"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,24]]},"references-count":41,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/adhm.202504458"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202504458","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,24]]},"assertion":[{"value":"2025-09-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e04458"}}