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To address this challenge, we developed an integrated bioadhesive strategy for suture\u2010free transplantation of biomimetic corneal constructs. Using digital light processing (DLP), we fabricated an epithelial\u2010stromal bilayer scaffold with spatially organized corneal epithelial cells (CECs) and corneal stromal stem cells (CSSCs) in their respective layers through photopolymerization of gelatin methacryloyl (GelMA)\/2\u2010aminoethyl methacrylated chondroitin sulfate (CSAMA) bioinks. Critically, N\u2010hydroxysuccinimide\u2010modified chondroitin sulfate methacrylate (CSMA\u2010NHS) was synthesized, and GelMA\u2010CSMA\u2010NHS bioadhesive achieved in situ anchoring of 3D bioprinted scaffolds to the corneal stroma through synergistic topological adhesion and amide\u2010bonding. The rabbit anterior lamellar keratoplasty model demonstrated that this combination strategy can achieve in situ corneal defect closure and effectively promote corneal re\u2010epithelialization and stromal regeneration, reducing corneal scar formation. This integrated strategy combines 3D bioprinting with in situ adhesion to simultaneously reconstruct the corneal multilayered architecture and enable suture\u2010free implantation, presenting a promising approach for corneal repair.<\/jats:p>","DOI":"10.1002\/adhm.202504578","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T13:59:43Z","timestamp":1767016783000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["3D\u2010Bioprinted Biomimetic Epithelial\u2010Stromal Hydrogel Construct with In Situ Photocrosslinkable Bioadhesive for Suture\u2010Free Corneal Regeneration"],"prefix":"10.1002","volume":"15","author":[{"given":"Xiongfeng","family":"Nie","sequence":"first","affiliation":[{"name":"School of Material Science and Engineering Tianjin Key Laboratory of Composite and Functional Materials Tianjin University  Tianjin 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