{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T05:54:34Z","timestamp":1781502874667,"version":"3.54.1"},"reference-count":65,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T00:00:00Z","timestamp":1770681600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T00:00:00Z","timestamp":1770681600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Plant\u2010based biomaterials, prized for their inherent biocompatibility and biodegradability, serve as excellent candidates for biomedical applications. This is largely due to the unique pharmacological properties of their bioactive metabolites. However, the sustainable and economical production of these metabolites, particularly from rare and endangered species, remains challenging. In this study, we developed a multicellular spheroid\u2013based cultivation strategy using medicinal plant cells to efficiently produce bioactive metabolites, exemplified by the endangered species\n                    <jats:italic>Saussurea involucrata<\/jats:italic>\n                    (SI). Optimized culture conditions stimulate sustainable production of SI\u2010derived secondary metabolites (SIM) enriched with antioxidant and anti\u2010inflammatory constituents. Single\u2010cell transcriptomics identify nine functionally specialized cell clusters responsible for the biosynthesis of pharmacologically active constituents. Ultra\u2010high performance liquid chromatography\u2010MS\/MS\u00a0catalogs 679 metabolites, with a chemical profile closely resembling that of wild SI. Network pharmacology links SIM to 318 therapeutic targets relevant to alcohol\u2010associated liver disease (ALD). In vitro, SIM decreases reactive oxygen species, suppresses hepatocyte apoptosis, and attenuates macrophage\u2010mediated inflammation. In an ALD mouse model, SIM administration alleviates hepatic lipid accumulation, restores liver function, and favorably remodels the gut microbiota. Together, these results establish a sustainable biomanufacturing paradigm for bioactive compounds from rare medicinal plants and highlight SIM as a promising candidate for ALD therapy.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202504623","type":"journal-article","created":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T06:46:56Z","timestamp":1770792416000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sustainable Cultivation of Rare and Endangered Medicinal Plant Multicellular Spheroids Producing Bioactive Therapeutics for Alcohol\u2010Related Liver Disease Therapy"],"prefix":"10.1002","volume":"15","author":[{"given":"Yixin","family":"Zhang","sequence":"first","affiliation":[{"name":"Ciechanover Institute of Precision and Regenerative Medicine School of Medicine The Chinese University of Hong Kong  Shenzhen China"},{"name":"Joint Laboratory of CUHKSZ\u2010Dalian Practical Biotechnology The Chinese University of Hong Kong  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Wang","sequence":"additional","affiliation":[{"name":"Shandong Key Laboratory of Digital Traditional Chinese Medicine Shandong Key Laboratory of Innovation and Application Research in Basic Theory of Traditional Chinese Medicine Shandong University of Traditional Chinese Medicine  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyang","family":"Wang","sequence":"additional","affiliation":[{"name":"Ciechanover Institute of Precision and Regenerative Medicine School of Medicine The Chinese University of Hong Kong  Shenzhen China"},{"name":"Joint Laboratory of CUHKSZ\u2010Dalian Practical Biotechnology The Chinese University of Hong Kong  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Liu","sequence":"additional","affiliation":[{"name":"Ciechanover Institute of Precision and Regenerative Medicine School of Medicine The Chinese University of Hong Kong  Shenzhen China"},{"name":"Joint Laboratory of CUHKSZ\u2010Dalian Practical Biotechnology The Chinese University of Hong Kong  Shenzhen Guangdong China"},{"name":"Dalian Practical Biotechnology Co. 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