{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:14:04Z","timestamp":1790612044936,"version":"4.1.0"},"reference-count":89,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T00:00:00Z","timestamp":1778025600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T00:00:00Z","timestamp":1778025600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HD114214"],"award-info":[{"award-number":["R01HD114214"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U01EB029132"],"award-info":[{"award-number":["U01EB029132"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The ascent of novel alternative methods in drug development spotlights the dual needs for improved biological fidelity to\u00a0in vivo, along with reproducibility, especially in regulatory applications. The need for pre\u2010clinical models of patient\u2010derived endometriosis lesions motivates the development of a vascularizable, completely synthetic extracellular matrix (v\u2010CS\u2010ECM) that supports morphogenesis of perfusable microvasculature in a microfluidic device, in the context of relevant lesion cells. This paper describes v\u2010CS\u2010ECM,\u00a0a peptide\u2010modified polyethylene glycol\u2010based hydrogel crosslinked with a cell\u2010degradable peptide that achieves these dual goals. Vessels form by morphogenesis after the liquid v\u2010CS\u2010ECM precursor, containing endothelial cells and fibroblasts, is injected into the tissue compartment to encapsulate cells. Vessel formation is influenced by ECM biochemical and biophysical properties, the source of vascular cells, and microphysiological system operating conditions. The v\u2010CS\u2010ECM also supports the co\u2010culture of endometrial epithelial organoids and fibroblasts, and formation of microvascularized endometriosis lesion\u2010like structures when all cell types are co\u2010encapsulated in a microfluidic device with constant flow. Hence, v\u2010CS\u2010ECM has the potential to improve preclinical evaluation of endometriosis drug efficacy by enabling microvascularized patient\u2010derived lesion models.<\/jats:p>","DOI":"10.1002\/adhm.202504936","type":"journal-article","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T09:09:09Z","timestamp":1778058549000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Development of a Synthetic Hydrogel to Foster Microvascularization of an Endometriosis Microphysiological System"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2121-1013","authenticated-orcid":false,"given":"Lauren","family":"Pruett","sequence":"first","affiliation":[{"name":"Department of Biological Engineering Massachusetts Institute of Technology  Cambridge Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2967-1353","authenticated-orcid":false,"given":"Laura","family":"Bahlmann","sequence":"additional","affiliation":[{"name":"Department of Biological Engineering Massachusetts Institute of Technology  Cambridge Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4185-7742","authenticated-orcid":false,"given":"Ryan","family":"Ogi","sequence":"additional","affiliation":[{"name":"Department of Biological Engineering Massachusetts Institute of Technology  Cambridge Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Jiao","sequence":"additional","affiliation":[{"name":"Department of Biological Engineering Massachusetts Institute of Technology  Cambridge Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7299-9306","authenticated-orcid":false,"given":"Priyatanu","family":"Roy","sequence":"additional","affiliation":[{"name":"Department of Biological Engineering Massachusetts Institute of Technology  Cambridge Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6517-4026","authenticated-orcid":false,"given":"Matthew","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering Massachusetts Institute of Technology  Cambridge Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Trumper","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering Massachusetts Institute of Technology  Cambridge Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1801-5548","authenticated-orcid":false,"given":"Linda","family":"Griffith","sequence":"additional","affiliation":[{"name":"Department of Biological Engineering Massachusetts Institute of Technology  Cambridge Massachusetts USA"},{"name":"Department of Mechanical Engineering Massachusetts Institute of Technology  Cambridge Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1LC00530H"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010bioeng\u2010090120\u2010094330"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/mi16101125"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2025.2975"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioaf164"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/aogs.13119"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioab183"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2021.103434"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43032\u2010023\u201001359\u2010w"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/smtd.202200143"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/SMMD.20220030"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202007199"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.3553237"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202303419"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2023.09.019"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3lc50210d"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.0c00640"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1063\/5.0097967"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2020.119921"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3839"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-02910-8"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1247125"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3312"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202400977"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2020.120231"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2017.03.030"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1039\/c6ib00245e"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2020.120125"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.medj.2023.07.004"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41563\u2010021\u201001085\u20101"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202000097"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44341\u2010025\u201000028\u20100"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2007.0314"},{"key":"e_1_2_10_35_1","first-page":"289","article-title":"Autocrine EGF Receptor Activation Mediates Endothelial Cell Migration and Vascular Morphogenesis Induced by VEGF under Interstitial Flow","volume":"312","author":"Semino C. E.","year":"2006","journal-title":"Experimental Cell Research"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010024\u201003376\u2010x"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2021.121032"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2013.0370"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2012.0430"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.4161\/cam.22680"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092\u20108674(89)90945\u20108"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2014.05.012"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1089\/biores.2013.0021"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2004.02.012"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/rb\/rbz003"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820250209"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.2019.33.1_supplement.525.7"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3IB40267C"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2024.122686"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3LC00512G"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202302561"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758\u20105090\/ab9316"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202206767"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2021.121248"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010023\u201001452\u20101"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3320"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10439\u2010018\u201002170\u20104"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13346\u2010021\u201000970\u20101"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41578-022-00447-8"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2025.111790"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2405474121"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsif.2025.0076"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/adbi.201600021"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21124526"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1067\/mva.2001.119401"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE\u201012\u20100147"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0131643"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201024013\u2010y"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1002\/btm2.10139"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2023.103610"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1LC00605C"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2022.110375"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-23644-5"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010017\u201000418\u20106"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4LC00460D"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202400567"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102010\u201012\u2010327338"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.00552"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1995.75.3.519"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1997.83.5.1711"},{"key":"e_1_2_10_81_1","first-page":"9","article-title":"Vascular Wall Shear Stress: Basic Principles and Methods","volume":"46","author":"Papaioannou T. G.","year":"2005","journal-title":"Hell J Cardiol HJC Hell Kardiologike Epitheorese"},{"key":"e_1_2_10_82_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.5036999"},{"key":"e_1_2_10_83_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma8041636"},{"key":"e_1_2_10_84_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aaz9499"},{"key":"e_1_2_10_85_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10439\u2010017\u20101797\u20105"},{"key":"e_1_2_10_86_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44294\u2010024\u201000020\u20104"},{"key":"e_1_2_10_87_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202303928"},{"key":"e_1_2_10_88_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2023.122121"},{"key":"e_1_2_10_89_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2025.1601420"},{"key":"e_1_2_10_90_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2021.105024"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202504936","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.202504936","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202504936","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T15:24:37Z","timestamp":1782401077000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202504936"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,6]]},"references-count":89,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/adhm.202504936"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202504936","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.10.06.680733","asserted-by":"object"}]},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,6]]},"assertion":[{"value":"2025-10-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e04936"}}