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In this study, Diels\u2010Alder click chemistry is employed as a dynamic\u2010covalent crosslinking method to create hydrogels composed of hyaluronic acid, gelatin, and PEG. By adjusting the pH during crosslinking, the tuneability of hydrogel stiffness and stability at pH values around the physiological pH of native cartilage is demonstrated. This pH modulation does not compromise hydrogel functionality, as encapsulated equine articular cartilage progenitor cell spheroids remain viable and functional for a culture period of 28 days. The hydrogel environment supports the deposition of cartilaginous extracellular matrix components, including collagens and sulphated glycosaminoglycans. Enhanced chondrogenesis and deposition of collagen type II are observed at higher spheroid concentrations, corresponding to inter\u2010spheroid distances of 100\u2013150\u00a0\u00b5m following hydrogel swelling, compared to lower concentrations at a distance of &gt;500\u00a0\u00b5m. To further improve construct robustness, the hydrogel constructs can be reinforced on day 1 with a melt electrowritten scaffold, increasing the compressive modulus 100\u2010fold by day 28 compared to non\u2010reinforced constructs, highlighting the potential of this system for engineering cartilage implants.<\/jats:p>","DOI":"10.1002\/adhm.202505013","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T12:29:35Z","timestamp":1771590575000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Diels\u2010Alder Click Chemistry as a Dynamic\u2010Covalent Crosslinking Method in Spheroid\u2010Encapsulating Hydrogels for Cartilage Engineering"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6732-2643","authenticated-orcid":false,"given":"Sanne M.","family":"van de Looij","sequence":"first","affiliation":[{"name":"Division of Pharmaceutics Utrecht Institute for Pharmaceutical Sciences (UIPS) Utrecht University  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia G.","family":"Vasilopoulou","sequence":"additional","affiliation":[{"name":"Department of Orthopedics University Medical Center Utrecht  Utrecht the Netherlands"},{"name":"Regenerative Medicine Center Utrecht  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lennard","family":"Spauwen","sequence":"additional","affiliation":[{"name":"Department of Orthopedics University Medical Center Utrecht  Utrecht the Netherlands"},{"name":"Regenerative Medicine Center Utrecht  Utrecht the Netherlands"},{"name":"HU University of Applied Sciences Utrecht  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoinette","family":"van den Dikkenberg","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutics Utrecht Institute for Pharmaceutical Sciences (UIPS) Utrecht University  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmijn V.","family":"Korpershoek","sequence":"additional","affiliation":[{"name":"Department of Orthopedics University Medical Center Utrecht  Utrecht the Netherlands"},{"name":"Department of Orthopedic Surgery Mayo Clinic Rochester  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mylene","family":"de Ruijter","sequence":"additional","affiliation":[{"name":"Department of Orthopedics University Medical Center Utrecht  Utrecht the Netherlands"},{"name":"Regenerative Medicine Center Utrecht  Utrecht the Netherlands"},{"name":"Department of Clinical Sciences Faculty of Veterinary Medicine Utrecht University  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos","family":"Malda","sequence":"additional","affiliation":[{"name":"Department of Orthopedics University Medical Center Utrecht  Utrecht the Netherlands"},{"name":"Regenerative Medicine Center Utrecht  Utrecht the Netherlands"},{"name":"Department of Clinical Sciences Faculty of Veterinary Medicine Utrecht University  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bas G. 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