{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T17:26:45Z","timestamp":1783013205905,"version":"3.54.6"},"reference-count":28,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82230073"],"award-info":[{"award-number":["82230073"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22372196"],"award-info":[{"award-number":["22372196"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The development of assembly interfaces that emulate subcellular chemical communication at the biotic\u2010abiotic interface is a central pursuit in synthetic biology. However, engineering in situ self\u2010assembling systems that combine robust retention against mucosal flushing and microenvironmental regulatory functions remains a formidable challenge. Herein, we present an intravesical in situ synthetic strategy that exploits endogenous microenvironmental triggers to construct a glycosaminoglycan (GAG)\u2010mimetic coating for durable mucosal repair. By leveraging catalase (CAT) and hydrogen peroxide (H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    ) inherent to inflammatory microenvironments, we drive the polymerization of alginate oligosaccharide\u2010conjugated polydopamine (AOS\u2010PDA). The resulting coating recapitulates the anionic and barrier properties while exhibiting anti\u2010inflammatory activity. In rat models of cyclophosphamide (CYP)\u2010induced interstitial cystitis, AOS\u2010PDA treatment significantly restored urothelial barrier integrity, reduced vascular permeability, and alleviated bladder dysfunction. Histological and molecular analyses confirmed the attenuation of inflammation, characterized by decreased mast cell infiltration and downregulation of key inflammatory mediators. Furthermore, the coating demonstrated sustained retention for up to three days post\u2010administration. These findings highlight a bio\u2010responsive, endogenous enzyme\u2010driven approach to synthesizing functional GAG\u2010like interfaces, offering a versatile therapeutic platform for broader mucosal repair applications.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202505017","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T13:07:06Z","timestamp":1769519226000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In Situ Synthesis of Glycosaminoglycan\u2010Mimicking Coatings Driven by Local Pathological Microenvironment: Boosting Bladder Mucosal Regeneration and Barrier Function"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3584-3193","authenticated-orcid":false,"given":"Huifang","family":"Du","sequence":"first","affiliation":[{"name":"Department of Anatomy Engineering Research Centre for Organ Intelligent Biological Manufacturing of Chongqing Key Lab for Biomechanics and Tissue Engineering of Chongqing Third Military Medical University  Chongqing China"},{"name":"Engineering Research Center of Tissue and Organ Regeneration and Manufacturing Ministry of Education  Chongqing China"},{"name":"College of Basic Medicine Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuming","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Anatomy Engineering Research Centre for Organ Intelligent Biological Manufacturing of Chongqing Key Lab for Biomechanics and Tissue Engineering of Chongqing Third Military Medical University  Chongqing China"},{"name":"Engineering Research Center of Tissue and Organ Regeneration and Manufacturing Ministry of Education  Chongqing China"},{"name":"Department of Urology Fourth Affiliated Hospital China Medical University  Shenyang 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanzhao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anatomy Engineering Research Centre for Organ Intelligent Biological Manufacturing of Chongqing Key Lab for Biomechanics and Tissue Engineering of Chongqing Third Military Medical University  Chongqing China"},{"name":"Engineering Research Center of Tissue and Organ Regeneration and Manufacturing Ministry of Education  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Anatomy Engineering Research Centre for Organ Intelligent Biological Manufacturing of Chongqing Key Lab for Biomechanics and Tissue Engineering of Chongqing Third Military Medical University  Chongqing China"},{"name":"Engineering Research Center of Tissue and Organ Regeneration and Manufacturing Ministry of Education  Chongqing 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Biological Manufacturing of Chongqing Key Lab for Biomechanics and Tissue Engineering of Chongqing Third Military Medical University  Chongqing China"},{"name":"Engineering Research Center of Tissue and Organ Regeneration and Manufacturing Ministry of Education  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuhong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Anatomy Engineering Research Centre for Organ Intelligent Biological Manufacturing of Chongqing Key Lab for Biomechanics and Tissue Engineering of Chongqing Third Military Medical University  Chongqing China"},{"name":"Engineering Research Center of Tissue and Organ Regeneration and Manufacturing Ministry of Education  Chongqing China"},{"name":"Burn and Combined Injury State Key Laboratory of Trauma  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji","family":"Zheng","sequence":"additional","affiliation":[{"name":"Burn and Combined Injury State Key Laboratory of Trauma  Chongqing China"},{"name":"Department of Urology Urologic Surgery Center Xinqiao Hospital Third Military Medical University (Army Medical University)  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youqian","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Anatomy Engineering Research Centre for Organ Intelligent Biological Manufacturing of Chongqing Key Lab for Biomechanics and Tissue Engineering of Chongqing Third Military Medical University  Chongqing China"},{"name":"Engineering Research Center of Tissue and Organ Regeneration and Manufacturing Ministry of Education  Chongqing 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