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Efficient isolation and expansion of ECFCs on polymeric surfaces either in vitro or in situ in the case of implants could significantly advance ECFC\u2010based therapies. We present a novel bifunctional surface modification strategy combining oriented antibodies and extracellular matrix\u2010derived peptides to selectively capture ECFCs on surfaces and then mediate firm adhesion, spreading and proliferation. Studies were conducted with anti\u2010CD309 antibodies and custom RGD peptides, previously shown to respectively enable ECFC capture and clonal expansion. To aid in oriented antibody immobilization on surfaces while modulating antibody\u2010RGD surface concentrations, Fc\u2010binding peptides and RGD peptides were co\u2010immobilized on aminated surfaces using click chemistry, followed by affinity\u2010mediated antibody immobilization. Bifunctional anti\u2010CD309 + RGD surfaces selectively captured ECFCs from a mixture with peripheral blood mononuclear cells in dynamic conditions. The presence of RGD peptides significantly enhanced cell spreading and proliferation, leading to additive effects on surface coverage under flow. Proof\u2010of\u2010concept studies demonstrated successful adaptation to 3D polystyrene microcarriers, showcasing potential scalability for clinical\u2010grade cell production. The surface modification scheme provides a versatile and clinically translatable platform for advancing ECFC\u2010based regenerative therapies.<\/jats:p>","DOI":"10.1002\/adhm.202505092","type":"journal-article","created":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T08:39:42Z","timestamp":1775723982000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Bifunctional Surfaces With Immobilized Antibodies and Bioactive Peptides Mediate Selective Capture and Proliferation of Endothelial Colony\u2010Forming Cells"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1432-7465","authenticated-orcid":false,"given":"Hugo A.","family":"Level","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering McGill University  Montreal 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