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To address this issue, we developed unique lanthanide complexes to act as modular probes that integrate the high\u2010sensitivity of TGL and deep\u2010tissue imaging capability of\u00a0\n                    <jats:sup>19<\/jats:sup>\n                    F magnetic resonance (MR). The probes are constructed from a multifunctional ligand, Mito\u2010BOTTA, comprising a mitochondria\u2010targeting 1\u2010(2\u2010aminoethyl)\u20104\u2010methylpyridinium group, a superoxide anion (O\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>\u2022\u2212<\/jats:sup>\n                    )\u2010responsive 3,5\u2010bis(trifluoromethyl)benzenesulfonyl (TFBS) moiety, and a terpyridine polyacid\u2010derived scaffold for coordination to Eu\n                    <jats:sup>3+<\/jats:sup>\n                    , Tb\n                    <jats:sup>3+<\/jats:sup>\n                    , or Gd\n                    <jats:sup>3+<\/jats:sup>\n                    . The coordination with Eu\n                    <jats:sup>3+<\/jats:sup>\n                    \/Tb\n                    <jats:sup>3+<\/jats:sup>\n                    \u2010mixture produces a ratiometric TGL probe Mito\u2010BOTTA\u2010Eu\n                    <jats:sup>3+<\/jats:sup>\n                    \/Tb\n                    <jats:sup>3+<\/jats:sup>\n                    that, upon O\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>\u2022\u2212<\/jats:sup>\n                    \u2010triggered cleavage of the TFBS moiety, exhibits a pronounced increase in Tb\n                    <jats:sup>3+<\/jats:sup>\n                    emission at 538\u00a0nm, accompanied by a decrease in Eu\n                    <jats:sup>3+<\/jats:sup>\n                    emission at 608\u00a0nm. This reciprocal spectral response enables precise and autofluorescence\u2010free ratiometric TGL imaging of mitochondrial O\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>\u2022\u2212<\/jats:sup>\n                    using the\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>538<\/jats:sub>\n                    \/\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>608<\/jats:sub>\n                    ratio as a quantitative readout. In parallel, the complexation with Gd\n                    <jats:sup>3+<\/jats:sup>\n                    yields Mito\u2010BOTTA\u2010Gd\n                    <jats:sup>3+<\/jats:sup>\n                    , where the paramagnetic Gd\n                    <jats:sup>3+<\/jats:sup>\n                    ion quenches the\n                    <jats:sup>19<\/jats:sup>\n                    F MR signal. The cleavage of TFBS in the presence of O\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>\u2022\u2212<\/jats:sup>\n                    releases the fluorinated TFBS moiety, which restores the\n                    <jats:sup>19<\/jats:sup>\n                    F MR signal, enabling a complementary \u201cturn\u2010on\u201d MR detection for O\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>\u2022\u2212<\/jats:sup>\n                    . Both probes demonstrated excellent mitochondrial localization, low cytotoxicity, and robust responsiveness to O\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>\u2022\u2212<\/jats:sup>\n                    in HepG2 cells as well as in murine models of alcohol\u2010 and CCl\n                    <jats:sub>4<\/jats:sub>\n                    \u2010induced acute liver injuries, which suggested the potential of the probes for dual\u2010mode TGL and\n                    <jats:sup>19<\/jats:sup>\n                    F MR bioimaging of O\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>\u2022\u2212<\/jats:sup>\n                    , offering a promising approach for the diagnostics of oxidative stress\u2010related diseases.\n                  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