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Although cartilage tissue engineering holds substantial promise, achieving reliable and efficient chondrogenesis remains a pressing challenge owing to the intrinsically quiescent phenotype of chondrocytes. We present engineered platelet\u2010derived extracellular vesicles (PEVs) that enhance auricular chondrocyte chondrogenesis in a 3D\u2010printed hydrogel, enabling safe and efficient ear regeneration. Initially, we demonstrated PEVs activated auricular chondrocytes, and sequencing identified miR\u201092a\u20103p as the key effector. Then we employed 3D bioprinting to fabricate a biomimetic ElaMA\/GelMA double\u2010network hydrogel auricular scaffold incorporating PEVs that were engineered to carry miRNA\u201092a\u20103p (miR@PEVs). In vivo, miR@PEV\u2010laden scaffolds exhibited the most robust chondrogenesis compared with PEVs and control groups; after one month of subcutaneous implantation, their Young's modulus approached that of native human auricular cartilage. The scaffolds also preserved satisfactory auricular morphology and displayed excellent biocompatibility. In vitro, miR@PEVs enhanced chondrocyte proliferation and migration, chiefly through miR\u201092a\u20103p\u2010mediated suppression of SMAD7 and subsequent activation of TGF\u03b2\/Smad signaling. Moreover, miR@PEVs promoted macrophage polarization toward the M2 phenotype, creating an immunological milieu conducive to cartilage formation. Altogether, integrating miR\u201092a\u20103p\u2010enriched miR@PEVs into the 3D\u2010printed ElaMA\/GelMA scaffold overcomes the key hurdles of auricular reconstruction and represents a promising strategy for total ear reconstruction.<\/jats:p>","DOI":"10.1002\/adhm.202505389","type":"journal-article","created":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T08:52:26Z","timestamp":1778403146000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Enhanced Auricular Cartilage Regeneration via 3D\u2010Printed Hydrogel With miR\u201092a\u20103p\u2010Enriched Platelet\u2010Rich Plasma\u2010Derived Extracellular Vesicles"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-8177-7792","authenticated-orcid":false,"given":"Shan","family":"Hua","sequence":"first","affiliation":[{"name":"Department of Plastic Surgery School of Medicine Shanghai East Hospital School of Medicine, Tongji University  Shanghai China"},{"name":"School of Medicine Tongji University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery School of Medicine Shanghai East Hospital School of Medicine, Tongji University  Shanghai China"},{"name":"School of Medicine Tongji University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiawei","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery School of Medicine Shanghai East Hospital School of Medicine, Tongji University  Shanghai China"},{"name":"School of Medicine Tongji University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery State Key Laboratory of Cardiology and Medical Innovation Center The Institute for Biomedical Engineering &amp; Nano Science Shanghai East Hospital, School of Medicine Tongji University  Shanghai P. 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