{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T06:51:31Z","timestamp":1791442291365,"version":"4.3.3"},"reference-count":51,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:00:00Z","timestamp":1773100800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:00:00Z","timestamp":1773100800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22375144"],"award-info":[{"award-number":["22375144"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32071384"],"award-info":[{"award-number":["32071384"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2102300"],"award-info":[{"award-number":["2021YFC2102300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["84434143D"],"award-info":[{"award-number":["84434143D"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Immune delivery and activation in lymph nodes (LNs) provide boosted cancer nanovaccine efficacy, but tumor\u2010induced immunosuppression in lymph nodes compromises nanovaccine efficacy. Toward that end, we engineered BIO\u2010GEM, a hierarchically structured biomimetic lymph node (bLN) platform comprising genipin\u2010crosslinked gelatin microspheres (GEM) encapsulating deformable albumin\u2010hitchhiking nanoemulsions (BIO, generated with bovine albumin, imiquimod adjuvant, and OVA antigen). Compared to conventional microparticles used for immune cell recruitment, BIO\u2010GEM forms antigen\u2010rich depots that better recruit antigen\u2010presenting cells (APCs) and T cells, creating an immunostimulatory niche for in situ T\u2010cell priming. Collagenase\u2010responsive degradation of GEM triggers sustained release of BIO, which targets LNs via the albumin\u2010hitchhiking pathway. This spatiotemporal delivery strategy synergizes bLN\u2010resident immune activation with LN\u2010directed antigen trafficking, yielding high CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u2010cell infiltration at injection sites, dendritic cell maturation, and elicitation of antigen\u2010specific cytotoxic T cells. In multiple B16 murine melanoma models, BIO\u2010GEM significantly suppressed tumor growth and extended the survival of mice. Intradermal vaccination was more efficacious than subcutaneous or intramuscular injection routes.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202505480","type":"journal-article","created":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T08:10:43Z","timestamp":1773130243000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Deformable Albumin\u2010Hitchhiking Nanocarriers Loaded in Gelatin Microspheres for Immune Cell Recruitment and Cancer Immunotherapy"],"prefix":"10.1002","volume":"15","author":[{"given":"He","family":"Ren","sequence":"first","affiliation":[{"name":"School of Synthetic Biology and Biomanufacturing State Key Laboratory of Synthetic Biology Frontiers Science Center for Synthetic Biology (Ministry of Education) Tianjin University  Tianjin P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Synthetic Biology and Biomanufacturing State Key Laboratory of Synthetic Biology Frontiers Science Center for Synthetic Biology (Ministry of Education) Tianjin University  Tianjin P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yizhen","family":"Du","sequence":"additional","affiliation":[{"name":"School of Synthetic Biology and Biomanufacturing State Key Laboratory of Synthetic Biology Frontiers Science Center for Synthetic Biology (Ministry of Education) Tianjin University  Tianjin P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Liaoning University  Shenyang Liaoning P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiexin","family":"Li","sequence":"additional","affiliation":[{"name":"School of Synthetic Biology and Biomanufacturing State Key Laboratory of Synthetic Biology Frontiers Science Center for Synthetic Biology (Ministry of Education) Tianjin University  Tianjin P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gengqi","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Synthetic Biology and Biomanufacturing State Key Laboratory of Synthetic Biology Frontiers Science Center for Synthetic Biology (Ministry of Education) Tianjin University  Tianjin P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan F.","family":"Lovell","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University at Buffalo State University of New York Buffalo New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6166-0470","authenticated-orcid":false,"given":"Yumiao","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Synthetic Biology and Biomanufacturing State Key Laboratory of Synthetic Biology Frontiers Science Center for Synthetic Biology (Ministry of Education) Tianjin University  Tianjin P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat4822"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202302756"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2017.52"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201703969"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-022-00699-x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adk2444"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202411361"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abb6981"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-06063-y"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-08508-4"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-24-0158"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12978"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41578-019-0110-7"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-023-00965-8"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.157.2.495"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091990206"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(01)07007-3"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100913-013254"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2623-z"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43018-021-00272-y"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41563-024-01961-6"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.euf.2016.09.003"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-34581-2"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/1878-0261.13047"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2024.101068"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2023.122420"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-019-0486-0"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3071"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201203859"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-018-0307-x"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202416260"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2821619"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200535730"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0706296105"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0753-3"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2868"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201808303"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202100106"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1414821112"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.309828"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1958.194.1.120"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20081811"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02454"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aas9930"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2022.122559"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.8b17555"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2016.04.012"},{"key":"e_1_2_10_49_1","first-page":"1377","article-title":"Why Certain Dyes are Useful for Localizing the Sentinel Lymph Node","volume":"43","author":"Tsopelas C.","year":"2002","journal-title":"Journal of Nuclear Medicine"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.4161\/hv.7.8.16274"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13346-010-0005-z"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.1c18117"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202505480","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.202505480","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202505480","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T06:15:53Z","timestamp":1791440153000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202505480"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,10]]},"references-count":51,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1002\/adhm.202505480"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202505480","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,10]]},"assertion":[{"value":"2025-11-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e05480"}}