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Strategies to rebuild functional marrow environments are essential to support hematopoietic stem cell (HSC) maintenance. However, the fundamental relationship between bone formation and bone marrow niche development, and how these processes are modulated by key biological variables, remains poorly understood. Here, we present an alginate\u2013gelatin cryogel platform that provides sustained BMP\u20102 release for ectopic osteogenesis and use it to systematically investigate the effects of BMP\u20102 dose, host immune status, and biological sex. Within 2\u20134 weeks, the cryogels supported the formation of a cortical bone shell and an internal trabecular bone network populated by hematopoietic tissue. Increasing the BMP\u20102 dose accelerated mineralization and doubled the resident HSC population, whereas immunocompromised hosts displayed a two\u2010week delay in niche development and a fourfold reduction in HSCs. Female mice exhibited enhanced niche formation relative to males under identical conditions. These findings establish BMP\u20102\u2010releasing hybrid click cryogels as a tunable bone marrow niche platform and highlight how host\u2010 and dose\u2010dependent parameters modulate engineered niche formation, providing insights that may inform future strategies to improve immune reconstitution following HSCT.<\/jats:p>","DOI":"10.1002\/adhm.202505821","type":"journal-article","created":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T09:14:37Z","timestamp":1771665277000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Kinetics of\n                    <i>De Novo<\/i>\n                    Bone and Bone Marrow Niche Formation With Hybrid Click Cryogels"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5070-0820","authenticated-orcid":false,"given":"Sangmin","family":"Lee","sequence":"first","affiliation":[{"name":"John A. 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