{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T04:27:52Z","timestamp":1786336072378,"version":"build-2736575974"},"reference-count":86,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T00:00:00Z","timestamp":1771718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T00:00:00Z","timestamp":1771718400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["PID2023\u2010146418OB\u2010I00"],"award-info":[{"award-number":["PID2023\u2010146418OB\u2010I00"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["PDC2025\u2010165423\u2010I00"],"award-info":[{"award-number":["PDC2025\u2010165423\u2010I00"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["MICIU\/AEI\/10.13039\/501100011033"],"award-info":[{"award-number":["MICIU\/AEI\/10.13039\/501100011033"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["ERDF"],"award-info":[{"award-number":["ERDF"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["EU"],"award-info":[{"award-number":["EU"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NCI R01 CA224605"],"award-info":[{"award-number":["NCI R01 CA224605"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NCI R01\u2010CA253615"],"award-info":[{"award-number":["NCI R01\u2010CA253615"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NCIR01\u2010CA253615\u201002S1Supplement"],"award-info":[{"award-number":["NCIR01\u2010CA253615\u201002S1Supplement"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Potato virus X (PVX), a filamentous, positive\u2010sense RNA plant virus, has been engineered into a molecular tool for diverse biotechnological applications, including cancer cell targeting. Here, we present the production and functional characterization of genetically\u2010encoded PVX\u2010derived nanoparticles decorated with nanobodies targeting two common receptors in human cancer cells, epidermal growth factor receptor (EGFR) and human epidermal growth factor 2 (HER2). We first generated a series of PVX\u2010derived nanoparticles displaying distinct nanobodies against EGFR and HER2 in\n                    <jats:italic>Nicotiana benthamiana<\/jats:italic>\n                    plants. Self\u2010assembly and structural integrity of the recombinant nanoparticles were confirmed by immunogold electron microscopy. We next characterized in vitro the cancer\u2010cell binding capacity of the different recombinant viral nanoparticles (VNPs) by flow cytometry and confocal microscopy. Select VNPs were further assayed in a pilot in vivo study using tumor\u2010bearing mice. Preliminary results showed that nanobody decoration can increase retention time and myeloid cell recruitment in the tumor microenvironment in HER2+ mouse tumor models in vivo. Nanobody\u2010displaying PVX\u2010derived nanoparticles may constitute a new plant\u2010produced biotechnological product for cancer immunotherapy.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202505936","type":"journal-article","created":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T06:35:18Z","timestamp":1771828518000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Plant\u2010Produced Viral Nanoparticles Decorated with Nanobodies Against HER2 Improve Retention and Recruitment of Immune Cells in Solid Tumors"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1655-9296","authenticated-orcid":false,"given":"Enrique","family":"Lozano\u2010Sanchez","sequence":"first","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Plantas Consejo Superior de Investigaciones Cient\u00edficas \u2013 Universitat Polit\u00e8cnica de Val\u00e8ncia  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1601-9369","authenticated-orcid":false,"given":"Miguel A.","family":"Moreno\u2010Gonzalez","sequence":"additional","affiliation":[{"name":"Aiiso Yufeng Li Family Department of Chemical and Nano Engineering University of California  San Diego California USA"},{"name":"Center for Nano\u2010ImmunoEngineering University of California  San Diego California USA"},{"name":"Moores Cancer Center University of California  San Diego California USA"},{"name":"Shu and K.C. Chien and Peter Farrell Collaboratory University of California  San Diego CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7786-0488","authenticated-orcid":false,"given":"Fernando","family":"Merwaiss","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Plantas Consejo Superior de Investigaciones Cient\u00edficas \u2013 Universitat Polit\u00e8cnica de Val\u00e8ncia  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0130-0481","authenticated-orcid":false,"given":"Nicole F.","family":"Steinmetz","sequence":"additional","affiliation":[{"name":"Aiiso Yufeng Li Family Department of Chemical and Nano Engineering University of California  San Diego California USA"},{"name":"Center for Nano\u2010ImmunoEngineering University of California  San Diego California USA"},{"name":"Moores Cancer Center University of California  San Diego California USA"},{"name":"Shu and K.C. Chien and Peter Farrell Collaboratory University of California  San Diego CA USA"},{"name":"Department of Bioengineering University of California San Diego California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6535-2889","authenticated-orcid":false,"given":"Jos\u00e9\u2010Antonio","family":"Dar\u00f2s","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Plantas Consejo Superior de Investigaciones Cient\u00edficas \u2013 Universitat Polit\u00e8cnica de Val\u00e8ncia  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.14344"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2024.108403"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/936940"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010021\u201001370\u20101"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41578\u2010021\u201000399\u20105"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44222\u2010023\u201000044\u20106"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.12412"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.4c13146"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277\u2010015\u20103867\u20103"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/wnan.1872"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nano.2018.09.004"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.9b02071"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsptsci.3c00285"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abf5375"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.7150\/ntno.19796"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21820"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010023\u201001668\u20101"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568\u2010024\u201000690\u2010x"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43018\u2010025\u201001042\u2010w"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/d41573\u2010025\u201000049\u20103"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.14230"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.25497"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2025.02.058"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23095253"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano9091329"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxrep.2025.102022"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.5C00100"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.5b04877"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.2217\/nnm.13.75"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201904958"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers11101609"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3762\/bjnano.11.28"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2025.113603"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/adtp.201800139"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2017.08.039"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6NR09099K"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12645\u2010018\u20100036\u20106"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12195\u2010015\u20100388\u20105"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3bm60277j"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/nl9035753"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589\u2010020\u20100502\u20104"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2019.00158"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.4c00507"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.4c07066"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1609"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573\u2010024\u201001081\u20105"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2016.1216742"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.26145"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2009.08.003"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0030149"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0288259"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201023948\u20106"},{"key":"e_1_2_10_55_1","first-page":"179","article-title":"In Vivo Properties of Three Human HER2\/Neu\u2010Expressing Murine Cell Lines in Immunocompetent Mice","volume":"49","author":"Penichet M. L.","year":"1999","journal-title":"Laboratory Animal Science"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.7b00107"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.2c06143"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201702151"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1021\/mp300240m"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.8b01365"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4TB00749B"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2017.06.006"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0BM00683A"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1021\/bc500545z"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.19187\/abc.20185290\u201095"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.0c00118"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25084327"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.877363"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014\u20105793(98)01586\u20105"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2017.01125"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abe4747"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573\u2010022\u201000579\u20100"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.127798"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adr8638"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature19946"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.7554\/ELIFE.105887.2"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262\u2010006\u20100180\u20104"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00259\u2010022\u201006066\u20103"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.1c00569"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S399322"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2013.05.008"},{"key":"e_1_2_10_82_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom13071042"},{"key":"e_1_2_10_83_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.7b05654"},{"key":"e_1_2_10_84_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201009\u20104672"},{"key":"e_1_2_10_85_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22012"},{"key":"e_1_2_10_86_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2013.10.014"},{"key":"e_1_2_10_87_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1318"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202505936","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.202505936","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202505936","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T03:29:38Z","timestamp":1786332578000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202505936"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,22]]},"references-count":86,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1002\/adhm.202505936"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202505936","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,22]]},"assertion":[{"value":"2025-12-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e05936"}}