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However, 3D neuronal cell culture is challenging due to their ultra\u2010soft nature and specific extracellular matrix (ECM) organization. This study presents a microfiber reinforcement approach, combining primary mouse spinal cord neurons (SCNs) in a purpose\u2010adaptable hyaluronic\u2010acid\u2010based matrix with melt electrowritten (MEW) frames to study disease mechanisms. The importance of laminins (LNs) is evaluated, which are vital for neuronal adhesion and maturation. Astrocytes (ACs) are mandatory in brain development and function by secretion of signal molecules, maintaining ion homeostasis, clearing of neurotransmitters, and by actively modulating neuronal activity. Three combinations are compared (i) isolated primary SCN, (ii) SCN with ACs (SCN\u2010AC) and (iii) SCN\u2010AC and LNs (SCN\u2010AC\u2010LN). Multimodal analysis by comparing protein expression, dendrite length, complex mechanical properties, and network functionality via Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010imaging allows validation of structural and functional neuronal network development. The suitability of the 3D model to study pathomechanisms is demonstrated for Stiff Person Syndrome (SPS). For the first time, functional impairment of spinal cord neurons by patient\u2010derived autoantibodies characteristic for SPS is recapitulated in a 3D spinal cord model platform adaptable to other disease types.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202505946","type":"journal-article","created":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T08:55:23Z","timestamp":1771145723000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Purpose\u2010Adaptable Reinforced 3D Hyaluronic\u2010Acid Based Platform to Study Pathomechanisms of the Central Nervous System"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-0905-2288","authenticated-orcid":false,"given":"Nicoletta","family":"Murenu","sequence":"first","affiliation":[{"name":"Institute for Clinical Neurobiology University Hospital of W\u00fcrzburg  W\u00fcrzburg 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