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While vascular organoids have shown potential, their clinical translation is limited by the need for invasive surgical resection to accommodate large 3D grafts. This study improves the vascular organoid technology pipeline by developing hPSC\u2010derived Pericyte\u2010Enriched Vascular Assemblies. These assemblies are composed of CD31\n                    <jats:sup>+<\/jats:sup>\n                    endothelial cells, PDGFR\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    pericytes, and SMA\u2010\u03b1\n                    <jats:sup>+<\/jats:sup>\n                    smooth muscle cells, with pericytes accounting for 62.6% of the composition, making them the key cells determining the vascular organoid phenotype. After transplanting single\u2010cell suspensions derived from these assemblies into the infarcted area, cerebral blood flow perfusion in mice was significantly restored, and sensory function improved. Mechanistic studies revealed that ischemic stress specifically upregulated the\n                    <jats:italic>PRKCA<\/jats:italic>\n                    signaling pathway in pericytes. Knockout of this kinase impaired the phenotype of the grafts, confirming its role as a master regulator of pericyte\u2010mediated vascular remodeling. This study shifts the paradigm from structural biomimicry to function\u2010driven design, highlighting the central regulatory role of pericytes in vascular organoid repair and providing new perspectives for cellular therapy and clinical treatment of ischemic stroke.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202600014","type":"journal-article","created":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T04:59:42Z","timestamp":1774501182000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transplantation of hPSC\u2010Derived Pericyte\u2010Enriched Vascular Assemblies Restores Cerebral Perfusion in the Context of Permanent Brain Ischemia"],"prefix":"10.1002","volume":"15","author":[{"given":"Jiru","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Neurosurgery Southwest Hospital Third Military Medical University (Army Medical University)  Chongqing China"},{"name":"Department of 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