{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T06:40:40Z","timestamp":1792737640146,"version":"4.5.0"},"reference-count":49,"publisher":"Wiley","issue":"39","license":[{"start":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:00:00Z","timestamp":1789862400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:00:00Z","timestamp":1789862400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Scaffold\u2010free tissue engineering enables the construction of biomimetic tissues and organs by preserving cell\u2013cell and cell\u2013matrix interactions while avoiding exogenous scaffolds and biomaterials. Yet current approaches are limited to thin sheets or simple spheroids and often lack cellular maturity and organized extracellular matrix (ECM). Here, anchored cell sheet engineering, a concept that previously introduced anchors to guide the remodeling of cell sheets into more mature fibers or sheets, is extended to achieve seamless, single\u2010step biofabrication of scaffold\u2010free hollow tubular and spherical constructs for sustained biological and mechanical functions under physiological conditions. Using custom culture devices with curved geometries for two\u2010dimensional (2D) culture, continuous confluent cell\u2010ECM layers were formed that were then delaminated and guided by strategically positioned central cores with different shapes and sizes to undergo tension\u2010mediated remodeling into mechanically stable hollow structures. This approach allows modulation of wall thickness, supports multilayered architectures, and yields constructs capable of withstanding fluid flow. By expanding scaffold\u2010free biofabrication beyond sheets and fibers to robust hollow geometries, this work establishes a versatile set of physiologically relevant building blocks for scalable bottom\u2010up assembly of complex, multi\u2010tissue organ\u2010like constructs within a bioassembloid framework.<\/jats:p>","DOI":"10.1002\/adhm.202600032","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T03:14:37Z","timestamp":1789960477000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Tissue Engineering Grail: Seamless Biofabrication of Scaffold\u2010Free Hollow Constructs"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8145-2556","authenticated-orcid":false,"given":"Alireza","family":"Shahin\u2010Shamsabadi","sequence":"first","affiliation":[{"name":"Evolved.Bio  Kitchener Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7893-1264","authenticated-orcid":false,"given":"John","family":"Cappuccitti","sequence":"additional","affiliation":[{"name":"Evolved.Bio  Kitchener Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,20]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3889"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/0975\u20107406.160013"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287\u2010015\u20100089\u2010y"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2019.00095"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1109\/TBME.2013.2243912"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3BM01934A"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.899657"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2015.00043"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/tri.13462"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaw7567"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2020.11.009"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41536-021-00133-3"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1063\/5.0225807"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOT.0000000000000690"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.12.084"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2023.0198"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smaim.2022.09.002"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287\u2010024\u201003937\u20104"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering10020211"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202308552"},{"issue":"4","key":"e_1_2_11_22_1","first-page":"420","article-title":"Tubular Bioartificial Organs: From Physiological Requirements to Fabrication Processes and Resulting Properties. 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