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However, the clinical translation is limited by its structural and functional immaturity. Recapitulating the structural organization of native myocardium is crucial for EHT maturation. Here, we introduce a novel biomimetic substrate derived from natural scales of the American shad. We identified that the parallel micro\u2010scale wrinkles on the scale surface possess a consistent width of \u223c25\u00a0\u00b5m, which closely matches the characteristic feature width of clinical adult human myocardium. When cultured on these scales, hiPSC\u2010CMs self\u2010organized into intact EHTs, exhibiting significant functional maturation, demonstrated by enhanced contractility, accelerated calcium handling kinetics, and a metabolic shift toward increased fatty acid oxidation. Furthermore, the hiPSC\u2010CM\/Scale platform reliably replicated adult\u2010like responses to cardiovascular drugs, highlighting its relevance for predictive pharmacology. In a mouse model of myocardial infarction, transplantation of the scale\u2010based EHTs as a cardiac patch improved cardiac function, reduced infarct size, promoted angiogenesis, and facilitated electrical integration within the host myocardium. Collectively, our findings establish shad scales as a unique and effective biomimetic platform for producing mature EHTs for therapeutic and drug screening applications.<\/jats:p>","DOI":"10.1002\/adhm.71031","type":"journal-article","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T08:26:22Z","timestamp":1773390382000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Natural Biomimetic Myocardial\u2010Matched Topography Promotes Engineered Heart Tissue Maturation for Cardiovascular Drug Evaluation and Myocardial Infarction Repair"],"prefix":"10.1002","volume":"15","author":[{"given":"Miao","family":"Xiao","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Surgery of the First Affiliated Hospital &amp; Institute For Cardiovascular Science State Key Laboratory of Radiation Medicine and Protection Suzhou Medical College Soochow University  Suzhou P. R. 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