{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T23:57:32Z","timestamp":1790639852812,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T00:00:00Z","timestamp":1779321600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T00:00:00Z","timestamp":1779321600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In the brain, proNGF, the NGF precursor, is in a homeostatic equilibrium with its processing product, mature NGF. Dysregulation of the NGF\/proNGF ratio has been associated with neurodegeneration in Alzheimer's disease (AD), positioning these neurotrophins as promising diagnostic biomarkers. Yet, their clinical validation as biomarkers has been hindered by the lack of analytical methods capable of discriminating and quantifying both isoforms under native conditions. Here, we introduce a dual electrochemical sensor based on Molecularly Imprinted Polymers (MIPs) that enables the simultaneous, selective, and label\u2010free quantification of NGF and proNGF. The sensors were fabricated via electropolymerization of\n                    <jats:italic>o<\/jats:italic>\n                    \u2010phenylenediamine on platinum microelectrodes, yielding highly specific recognition sites for each isoform. The MIP\u2010based platform demonstrates remarkable selectivity, reproducibility, and isoform discrimination, achieving picomolar detection limits even for NGF, which is typically present at low concentration in cerebrospinal fluid (CSF). Validated on clinical CSF samples from AD and control patients, this system successfully quantifies both NGF and proNGF without antibodies or sample denaturation. To the best of our knowledge, this represents the first quantitative and simultaneous detection of NGF and proNGF under native conditions. This technology paves the way toward cost\u2010effective, high\u2010throughput, and point\u2010of\u2010care diagnostics for Alzheimer's and other neurodegenerative diseases.\n                  <\/jats:p>","DOI":"10.1002\/adhm.71262","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T06:20:34Z","timestamp":1779430834000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Simultaneous Electrochemical Detection of NGF and proNGF Under Native Conditions Using Molecularly Imprinted Polymers: Toward Point\u2010of\u2010Care Diagnosis of Alzheimer's Disease"],"prefix":"10.1002","volume":"15","author":[{"given":"Giulia","family":"Siciliano","sequence":"first","affiliation":[{"name":"CNR NANOTEC \u2013 Institute of Nanotechnology  Lecce Italy"},{"name":"TecnoMedPuglia Tecnopolo Per La Medicina Di Precisione (Biotech Lecce Hub) c\/o Campus Ecotekne  Lecce Italy"},{"name":"Department of Experimental Medicine University of Salento  Lecce Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfredo De","family":"Cillis","sequence":"additional","affiliation":[{"name":"CNR NANOTEC \u2013 Institute of Nanotechnology  Lecce Italy"},{"name":"TecnoMedPuglia Tecnopolo Per La Medicina Di Precisione (Biotech Lecce Hub) c\/o Campus Ecotekne  Lecce Italy"},{"name":"Department of Innovation Engineering University of Salento  Lecce Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Clabassi","sequence":"additional","affiliation":[{"name":"CNR NANOTEC \u2013 Institute of Nanotechnology  Lecce Italy"},{"name":"TecnoMedPuglia Tecnopolo Per La Medicina Di Precisione (Biotech Lecce Hub) c\/o Campus Ecotekne  Lecce Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Ferrara","sequence":"additional","affiliation":[{"name":"CNR NANOTEC \u2013 Institute of Nanotechnology  Lecce Italy"},{"name":"TecnoMedPuglia Tecnopolo Per La Medicina Di Precisione (Biotech Lecce Hub) c\/o Campus Ecotekne  Lecce Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Serena","family":"Chiriac\u00f2","sequence":"additional","affiliation":[{"name":"CNR NANOTEC \u2013 Institute of Nanotechnology  Lecce Italy"},{"name":"TecnoMedPuglia Tecnopolo Per La Medicina Di Precisione (Biotech Lecce Hub) c\/o Campus Ecotekne  Lecce Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Turco","sequence":"additional","affiliation":[{"name":"CNR NANOTEC \u2013 Institute of Nanotechnology  Lecce Italy"},{"name":"TecnoMedPuglia Tecnopolo Per La Medicina Di Precisione (Biotech Lecce Hub) c\/o Campus Ecotekne  Lecce Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clarissa","family":"Loiola","sequence":"additional","affiliation":[{"name":"European Brain Research Institute (EBRI) Rita Levi\u2010Montalcini  Lecce Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Zecca","sequence":"additional","affiliation":[{"name":"Center For Neurodegenerative Diseases and the Aging Brain Department of Clinical Research in\u00a0Neurology University of Bari \u201cAldo Moro\u201d; \u201cPia Fondazione Cardinale G. Panico\u201d, Tricase, Lecce, Italy Lecce Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Teresa","family":"Dell'Abate","sequence":"additional","affiliation":[{"name":"Center For Neurodegenerative Diseases and the Aging Brain Department of Clinical Research in\u00a0Neurology University of Bari \u201cAldo Moro\u201d; \u201cPia Fondazione Cardinale G. Panico\u201d, Tricase, Lecce, Italy Lecce Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giancarlo","family":"Logroscino","sequence":"additional","affiliation":[{"name":"Center For Neurodegenerative Diseases and the Aging Brain Department of Clinical Research in\u00a0Neurology University of Bari \u201cAldo Moro\u201d; \u201cPia Fondazione Cardinale G. 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