{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T16:52:57Z","timestamp":1786121577920,"version":"build-2736575974"},"reference-count":63,"publisher":"Wiley","issue":"29","license":[{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Zinc (Zn)\u2010based metals have been considered as promising materials for bone regeneration and repair. Neutrophils, as the initial responders in the acute inflammatory phase, play a critical role by generating specialized mediators that facilitate the natural resolution of inflammation. Nevertheless, the mechanisms governing neutrophil\u2010driven inflammation following the implantation of Zn\u2010based metals are not fully elucidated. This study investigated neutrophil\u2010macrophage crosstalk around Zn\u2010based implants using single\u2010cell transcriptomic profiling. A high\u2010resolution cellular atlas of the peri\u2010implant bone marrow microenvironment was generated in a rat femoral model comparing Zn\u2010based implants with the titanium (Ti) ones. The results revealed that Zn implants induced a distinct neutrophil (Neu) subset heterogeneity, including\n                    <jats:italic>Cd177<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    Neu,\n                    <jats:italic>Ifit1bl<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    Neu,\n                    <jats:italic>RatNP\u20103b<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    Neu, and\n                    <jats:italic>Prtn3<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    Neu, the latter demonstrating enhanced tissue repair ability through osteoclast differentiation pathways. Zn\u2010based metallic implants reduced macrophage (Mac) reactivity and promoted\n                    <jats:italic>\n                      Hp\n                      <jats:sup>+<\/jats:sup>\n                    <\/jats:italic>\n                    Mac dominance compared to Ti implants, which showed higher\n                    <jats:italic>\n                      Apoe\n                      <jats:sup>+<\/jats:sup>\n                    <\/jats:italic>\n                    Mac proportions. Crosstalk analysis identified a significant interaction between the neutrophil subset\n                    <jats:italic>Ifit1bl<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    Neu and the macrophage subset\n                    <jats:italic>Eno3<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    Mac under Zn implantation, mediated by the Lgals9\u2010Ighm ligand\u2010receptor axis. These findings suggested that degradation products of Zn\u2010based implants modulated immune cell differentiation and interaction networks, thereby modulating biological response.\n                  <\/jats:p>","DOI":"10.1002\/adhm.71274","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T13:15:12Z","timestamp":1784034912000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Neutrophil\u2010Mediated Inflammatory Response to Zinc Bone Implants: A Single\u2010Cell Transcriptomic Landscape"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2237-9918","authenticated-orcid":false,"given":"Mi","family":"Du","sequence":"first","affiliation":[{"name":"Department of Implantology School and Hospital of Stomatology Cheeloo College of Medicine Shandong University  Jinan China"},{"name":"Shandong Key Laboratory of Oral Diseases Shandong University  Jinan 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 Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiannan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics School and Hospital of Stomatology Guangzhou Medical University  Guangzhou China"},{"name":"Guangdong Engineering Research Center of Oral Restoration and Reconstruction School and Hospital of Stomatology Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0782-6713","authenticated-orcid":false,"given":"Ping","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics School and Hospital of Stomatology Guangzhou Medical University  Guangzhou China"},{"name":"Guangdong Engineering Research Center of Oral Restoration and Reconstruction School and Hospital of Stomatology Guangzhou Medical University  Guangzhou China"},{"name":"Department of Oral Surgery Sechenov First Moscow State Medical 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