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Here, we present a stereo microfluidic well array\u2010based hepatocellular carcinoma\u2010on\u2010a\u2010chip for one\u2010stop individualized drug evaluation. We design the device with two modules for tumor spheroid formation and drug concentration gradient generation in a single platform. Using projection micro\u2010stereolithography, the chip template is fabricated in a one\u2010step process and subsequently molded in polydimethylsiloxane (PDMS). Controlled microfluidic flow in the chip enables precise cell loading into microwells, producing uniform\u2010sized and high\u2010viability HCC spheroids (\u223c2200 per chip) under dynamic culture conditions. Based on these features, this tumor\u2010on\u2010a\u2010chip further supports high\u2010throughput drug screening across tunable and multiple concentration gradients, validated with patient\u2010derived HCC samples. Thus, this platform is capable of high\u2010throughput generation of three\u2010dimensional spheroids and multi\u2010dose drug gradient exposure; it has already completed proof\u2010of\u2010concept studies for a drug screening platform based on microfluidic spheroids, laying the groundwork for subsequent translational research incorporating clinical data.<\/jats:p>","DOI":"10.1002\/adhm.71327","type":"journal-article","created":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T04:20:24Z","timestamp":1780546824000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hepatocellular Carcinoma\u2010on\u2010a\u2010Chip Based on Microfluidic Well Array for Personalized Drug Evaluation"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3151-7205","authenticated-orcid":false,"given":"Sunhao","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Immunology 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