{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T16:52:57Z","timestamp":1786121577953,"version":"build-2736575974"},"reference-count":65,"publisher":"Wiley","issue":"29","license":[{"start":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T00:00:00Z","timestamp":1782518400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T00:00:00Z","timestamp":1782518400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP220103135"],"award-info":[{"award-number":["DP220103135"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["LP220100450"],"award-info":[{"award-number":["LP220100450"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["IH220100017"],"award-info":[{"award-number":["IH220100017"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Pretargeted radiotheranostics decouple tumor recognition from radionuclide delivery and utilize in vivo click chemistry to achieve targeted delivery of radioactive payloads. A major barrier to efficient radionuclide delivery via the pretargeting approach is the suboptimal pharmacokinetics of the small\u2010molecule radioligand, which rapid clearance restricts the in vivo reaction window for bioorthogonal capture, thereby also limiting clinical translation. Herein, we investigated an in situ albumin engagement strategy to modulate the pharmacokinetics of a clickable radioligand in a PSMA\u2010targeted, polymer\u2010assisted bispecific antibody pretargeting system. By incorporating an albumin binder into the small\u2010molecule radioligand, systemic circulation was prolonged to expand the bioorthogonal capture window, without perturbing target affinity or receptor biology. This design may offer a modular advantage, as the clickable radioligand can be optimized independently while different pretargeting agents may, in principle, be adapted for alternative receptor systems. Within the PSMA\u2010targeting BsAb\/HBP system, the lead candidate (DOTA\u2010mTz\u2010\n                    <jats:bold>s<\/jats:bold>\n                    ALB), compared to the non\u2010albumin binding construct, increased tumor\u2010associated uptake from \u223c1.3 to \u223c4.7%ID g\n                    <jats:sup>\u22121<\/jats:sup>\n                    (\u223c3.5\u2010fold increase) and improved tumor\u2010to\u2010liver ratios from \u223c0.1 to \u223c0.6 (\u223c6\u2010fold increase), while systemic background largely resolves within 24\u201348\u00a0h. Together, these results support albumin engagement as a modular pharmacokinetic\u2010tuning element to improve pretargeted radiotheranostic delivery.\n                  <\/jats:p>","DOI":"10.1002\/adhm.71385","type":"journal-article","created":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T08:26:29Z","timestamp":1782548789000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Affinity\u2010Tuned Albumin Hitchhiking Extends the Bioorthogonal Capture Window in Pretargeting Radiotheranostics"],"prefix":"10.1002","volume":"15","author":[{"given":"Xie","family":"He","sequence":"first","affiliation":[{"name":"Australian Institute for Bioengineering and Nanotechnology and Centre for Advanced Imaging University of Queensland  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas L.","family":"Fletcher","sequence":"additional","affiliation":[{"name":"Australian Institute for Bioengineering and Nanotechnology and Centre for Advanced Imaging University of Queensland  Brisbane Queensland Australia"},{"name":"ARC Research Hub for Advanced Manufacture of Targeted Radiopharmaceuticals (AMTAR) The University of Queensland  St Lucia Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gayathri R.","family":"Ediriweera","sequence":"additional","affiliation":[{"name":"Australian Institute for Bioengineering and Nanotechnology and Centre for Advanced Imaging University of Queensland  Brisbane Queensland Australia"},{"name":"ARC Research Hub for Advanced Manufacture of Targeted Radiopharmaceuticals (AMTAR) The University of Queensland  St Lucia Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifei","family":"Zhu","sequence":"additional","affiliation":[{"name":"Australian Institute for Bioengineering and Nanotechnology and Centre for Advanced Imaging University of Queensland  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Humphries","sequence":"additional","affiliation":[{"name":"Australian Institute for Bioengineering and Nanotechnology and Centre for Advanced Imaging University of Queensland  Brisbane Queensland Australia"},{"name":"ARC Research Hub for Advanced Manufacture of Targeted Radiopharmaceuticals (AMTAR) The University of Queensland  St Lucia Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher B.","family":"Howard","sequence":"additional","affiliation":[{"name":"Australian Institute for Bioengineering and Nanotechnology and Centre for Advanced Imaging University of Queensland  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiazheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Australian Institute for Bioengineering and Nanotechnology and Centre for Advanced Imaging University of Queensland  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig A.","family":"Bell","sequence":"additional","affiliation":[{"name":"Australian Institute for Bioengineering and Nanotechnology and Centre for Advanced Imaging University of Queensland  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristian","family":"Kempe","sequence":"additional","affiliation":[{"name":"Drug Delivery, Disposition and Dynamics Monash Institute of Pharmaceutical Sciences Monash University  Parkville Victoria Australia"},{"name":"Materials Science and Engineering Monash University  Clayton Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristofer J.","family":"Thurecht","sequence":"additional","affiliation":[{"name":"Australian Institute for Bioengineering and Nanotechnology and Centre for Advanced Imaging University of Queensland  Brisbane Queensland Australia"},{"name":"ARC Research Hub for Advanced Manufacture of Targeted Radiopharmaceuticals (AMTAR) The University of Queensland  St Lucia Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23095023"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571\u2010022\u201000652\u2010y"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.116.186940"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202402361"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.CCR\u201007\u20105093"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00259\u2010010\u20101433\u20101"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2013.09.015"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.2c00760"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.4c01193"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277\u2010012\u20100367\u20106"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.3582"},{"issue":"3","key":"e_1_2_8_13_1","first-page":"400","article-title":"Pretargeted Radioimmunotherapy of Cancer: Progress Step by Step","volume":"44","author":"Boerman O. C.","year":"2003","journal-title":"Journal of Nuclear Medicine"},{"issue":"8","key":"e_1_2_8_14_1","first-page":"1294","article-title":"Investigations of Avidin and Biotin for Imaging Applications","volume":"28","author":"Hnatowich D. J.","year":"1987","journal-title":"Journal of Nuclear Medicine"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/anse.202300053"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlcr.3149"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200906294"},{"key":"e_1_2_8_18_1","unstructured":"ClinicalTrials.gov. PET Imaging Using 64Cu\u2010Tz\u2010SarAr and hu5B1\u2010TCO in People With Pancreatic Colorectal Bladder Cancer or Cancers With Elevated CA199 https:\/\/clinicaltrials.gov\/study\/NCT05737615."},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201600263"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano10122452"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.9b10304"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-4402-7_5"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/1099\u20100518(20000715)38:14<2505::AID\u2010POLA10>3.0.CO;2\u20108"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0SC00078G"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2009960117"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.01476"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9MD00018F"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205854200"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2013.05.005"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15174259"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.18719"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.112.107235"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2025.113757"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.6b00487"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200704936"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.8b00341"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00259\u2010018\u20104004\u20105"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.5c02195"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.116.188722"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25112542"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.118.221150"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28166158"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nucmedbio.2024.108911"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2026.114708"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.5c01157"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8CC05831H"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2CC02443H"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2015.09.012"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2020.02.019"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201015051\u2010y"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-24-1060"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4PY00999A"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.3c00138"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.5b00294"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2023.103738"},{"key":"e_1_2_8_56_1","volume-title":"24\/30475247 DC: BS EN ISO 10993\u20134:2017\/Amd 1 Biological Evaluation of Medical Devices: Part 4. Selection of Tests for Interactions With Blood. Amendment 1","author":"British Standards Institute","year":"2024"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nucmedbio.2017.09.001"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.108.053249"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00155"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2025.1546465"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2008.10.004"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.5b00913"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000091655.77601.0c"},{"key":"e_1_2_8_64_1","unstructured":"N. H.Bander \u201cTreatment and Diagnosis of Prostate Cancer With Antibodies to Extracellur PSMA Domains \u201d US6107090A (US Patent Office 2000)."},{"issue":"13","key":"e_1_2_8_65_1","first-page":"3192","article-title":"Five Different Anti\u2010Prostate\u2010Specific Membrane Antigen (PSMA) Antibodies Confirm PSMA Expression in Tumor\u2010Associated Neovasculature","volume":"59","author":"Chang S. S.","year":"1999","journal-title":"Cancer Research"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1021\/bc2002715"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.71385","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.71385","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.71385","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T05:41:32Z","timestamp":1786081292000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.71385"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,27]]},"references-count":65,"journal-issue":{"issue":"29","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/adhm.71385"],"URL":"https:\/\/doi.org\/10.1002\/adhm.71385","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,27]]},"assertion":[{"value":"2026-03-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71385"}}