{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T16:52:57Z","timestamp":1786121577976,"version":"build-2736575974"},"reference-count":66,"publisher":"Wiley","issue":"29","license":[{"start":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T00:00:00Z","timestamp":1784419200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T00:00:00Z","timestamp":1784419200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["101150675"],"award-info":[{"award-number":["101150675"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Anisotropic biomaterials are broadly studied in regenerative medicine as they aim to mimic the hierarchical structures of native tissue. The Anisogel, an anisotropic microgel\u2010in\u2010hydrogel system, involves two key factors for tissue engineering: guidance cues\u2014magnetically aligned rod\u2010shape structures\u2014and a surrounding matrix\u2014polyethylene glycol\u2010vinylsulfone (PEG\u2010VS) with a degradable peptide crosslinker. This research shows the potential of using a PEG\u2010VS\u2010 based Anisogel to allow for guided ingrowth and chondrogenic differentiation of human mesenchymal stromal cells (hMSCs) modulated by the alignment and size of anisometric microgels. The properties of the surrounding matrix are optimized for the availability of anchoring peptides (RGD concentration) and matrix density (hydrogel precursor concentration). The microgels of highest tested dimensions (10 \u00d7 10 \u00d7 100\u00a0\u00b5m\n                    <jats:sup>3<\/jats:sup>\n                    ) lead to a donor\u2010independent significant upregulation of chondrogenic markers (\n                    <jats:italic>SOX 9<\/jats:italic>\n                    ,\n                    <jats:italic>ACAN<\/jats:italic>\n                    and\n                    <jats:italic>COL2A1<\/jats:italic>\n                    ) and a decrease of hypertrophic chondrogenic markers (\n                    <jats:italic>RUNX 2, COL10A1<\/jats:italic>\n                    ), compared to unaligned and smaller microgel sizes. As a proof of concept, the Anisogel is tested in a semi\u2010orthotopic mouse model where the effect of microgel alignment on cell infiltration and osteochondral tissue formation is evaluated.\n                  <\/jats:p>","DOI":"10.1002\/adhm.71393","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T04:47:39Z","timestamp":1784522859000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Structural Guidance in 3D Microgel\u2010in\u2010Hydrogel Systems to Improve Chondrogenesis"],"prefix":"10.1002","volume":"15","author":[{"given":"Julia","family":"Kamp","sequence":"first","affiliation":[{"name":"DWI \u2013 Leibniz\u2010Institute for Interactive Materials  Aachen Germany"},{"name":"Institute of Technical and Macromolecular Chemistry (ITMC), Chair for Macromolecular Materials for Medicine RWTH University Aachen  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alisa C.","family":"Suturin","sequence":"additional","affiliation":[{"name":"DWI \u2013 Leibniz\u2010Institute for Interactive Materials  Aachen Germany"},{"name":"Institute of Technical and Macromolecular Chemistry (ITMC), Chair for Macromolecular Materials for Medicine RWTH University Aachen  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia A.","family":"Garrido","sequence":"additional","affiliation":[{"name":"DWI \u2013 Leibniz\u2010Institute for Interactive Materials  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaole","family":"Tong","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics &amp; Sports Medicine, Erasmus MC University Medical Center Rotterdam  Rotterdam Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jietao","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics &amp; Sports Medicine, Erasmus MC University Medical Center Rotterdam  Rotterdam Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Kops","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics &amp; Sports Medicine, Erasmus MC University Medical Center Rotterdam  Rotterdam Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lea\u2010Sophie","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"DWI \u2013 Leibniz\u2010Institute for Interactive Materials  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Till","family":"H\u00fclsmann","sequence":"additional","affiliation":[{"name":"DWI \u2013 Leibniz\u2010Institute for Interactive Materials  Aachen Germany"},{"name":"Max Planck School Matter to Life  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdolrahman","family":"Omidinia\u2010Anarkoli","sequence":"additional","affiliation":[{"name":"DWI \u2013 Leibniz\u2010Institute for Interactive Materials  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Klasen","sequence":"additional","affiliation":[{"name":"DWI \u2013 Leibniz\u2010Institute for Interactive Materials  Aachen Germany"},{"name":"Institute of Technical and Macromolecular Chemistry (ITMC), Chair for Macromolecular Materials for Medicine RWTH University Aachen  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7095-4358","authenticated-orcid":false,"given":"Tam\u00e1s","family":"Haraszti","sequence":"additional","affiliation":[{"name":"DWI \u2013 Leibniz\u2010Institute for Interactive Materials  Aachen Germany"},{"name":"Institute of Technical and Macromolecular Chemistry (ITMC), Chair for Macromolecular Materials for Medicine RWTH University Aachen  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerjo J. 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