{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T10:52:21Z","timestamp":1791543141148,"version":"4.3.4"},"reference-count":95,"publisher":"Wiley","issue":"37","license":[{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN\u20102021\u201003960"],"award-info":[{"award-number":["RGPIN\u20102021\u201003960"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["DGECR\u20102021\u201000337"],"award-info":[{"award-number":["DGECR\u20102021\u201000337"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Dilated cardiomyopathy (DCM) is the leading cause of heart transplantation, with a 50% risk of progression to heart failure within 5 years. Conventional disease modeling approaches fail to recapitulate the sophisticated function of the human heart. Alternatively, heart\u2010on\u2010a\u2010chip (HOC) platforms enable real\u2010time monitoring of disease progression and drug responses using miniaturized engineered heart tissues. Here, we developed a functional HOC model using patient\u2010specific human induced pluripotent stem cells (hiPSCs), reprogrammed from the patients\u2019 blood samples. The chip contains two cell\u2010seeding chambers with flexible silicone pillars to support tissue formation. Healthy and DCM hiPSCs were differentiated into cardiomyocytes, combined with an optimized ratio of human cardiac fibroblasts, encapsulated in a fibrin\/Geltrex hydrogel (containing fluorescent beads), and seeded in the device chambers. The tissue gradually compacted and started beating spontaneously. Immunofluorescence assay revealed structural abnormalities in DCM tissues, including reduced cell alignment and elongation. The tissue functional responses (e.g., calcium transients and beating) were investigated after 2 weeks of culture, revealing arrhythmia\u2010like behavior in DCM tissue and highlighting functional hallmarks of the disease. Finally, the platform was validated using norepinephrine to assess the functional responsiveness of the tissues. These results demonstrate the potential of this system for disease modeling and future patient\u2010specific investigations.<\/jats:p>","DOI":"10.1002\/adhm.71464","type":"journal-article","created":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T10:53:41Z","timestamp":1784976821000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Patient\u2010Derived 3D Heart\u2010On\u2010a\u2010Chip Model of Dilated Cardiomyopathy With Embedded Bead\u2010Based Mapping of Tissue Contractility"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0542-4995","authenticated-orcid":false,"given":"Ali","family":"Mousavi","sequence":"first","affiliation":[{"name":"Institute of Biomedical Engineering Department of Pharmacology and Physiology Faculty of Medicine Universit\u00e9 De Montr\u00e9al  Montr\u00e9al QC Canada"},{"name":"CHU Sainte\u2010Justine Research Center  Montr\u00e9al QC Canada"},{"name":"Montr\u00e9al TransMedTech Institute  Montr\u00e9al QC Canada"},{"name":"Research Institute of the McGill University Health Centre  Montr\u00e9al QC Canada"},{"name":"Surgical and Interventional Sciences, Department of Surgery Faculty of Medicine and Health Sciences McGill University  Montr\u00e9al QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8084-8823","authenticated-orcid":false,"given":"Ludovic","family":"Mouttet","sequence":"additional","affiliation":[{"name":"Research Institute of the McGill University Health Centre  Montr\u00e9al QC Canada"},{"name":"Surgical and Interventional Sciences, Department of Surgery Faculty of Medicine and Health Sciences McGill University  Montr\u00e9al QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shihao","family":"Cui","sequence":"additional","affiliation":[{"name":"CHU Sainte\u2010Justine Research Center  Montr\u00e9al QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0785-5285","authenticated-orcid":false,"given":"Yasaman","family":"Hekmatnia","sequence":"additional","affiliation":[{"name":"Research Institute of the McGill University Health Centre  Montr\u00e9al QC Canada"},{"name":"Surgical and Interventional Sciences, Department of Surgery Faculty of Medicine and Health Sciences McGill University  Montr\u00e9al QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0406-8670","authenticated-orcid":false,"given":"Mehran","family":"Mottahedi","sequence":"additional","affiliation":[{"name":"Research Institute of the McGill University Health Centre  Montr\u00e9al QC Canada"},{"name":"Surgical and Interventional Sciences, Department of Surgery Faculty of Medicine and Health Sciences McGill University  Montr\u00e9al QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ida","family":"Derish","sequence":"additional","affiliation":[{"name":"Research Institute of the McGill University Health Centre  Montr\u00e9al QC Canada"},{"name":"Surgical and Interventional Sciences, Department of Surgery Faculty of Medicine and Health Sciences McGill University  Montr\u00e9al QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1240-1568","authenticated-orcid":false,"given":"Naimeh","family":"Rafatian","sequence":"additional","affiliation":[{"name":"CHU Sainte\u2010Justine Research Center  Montr\u00e9al QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Aurousseau","sequence":"additional","affiliation":[{"name":"eNUVIO Inc.  Montr\u00e9al QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregor","family":"Andelfinger","sequence":"additional","affiliation":[{"name":"CHU Sainte\u2010Justine Research Center  Montr\u00e9al QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renzo","family":"Cecere","sequence":"additional","affiliation":[{"name":"Research Institute of the McGill University Health Centre  Montr\u00e9al QC Canada"},{"name":"Surgical and Interventional Sciences, Department of Surgery Faculty of Medicine and Health Sciences McGill University  Montr\u00e9al QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5596-673X","authenticated-orcid":false,"given":"Houman","family":"Savoji","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering Department of Pharmacology and Physiology Faculty of Medicine Universit\u00e9 De Montr\u00e9al  Montr\u00e9al QC Canada"},{"name":"CHU Sainte\u2010Justine Research Center  Montr\u00e9al QC Canada"},{"name":"Montr\u00e9al TransMedTech Institute  Montr\u00e9al QC Canada"},{"name":"Department of Mechanical Engineering Polytechnique Montr\u00e9al  Montr\u00e9al QC Canada"},{"name":"Institute of Biomedical Engineering Polytechnique Montr\u00e9al  Montr\u00e9al QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/1440\u20101681.70007"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.309711"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2015.12.014"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10741\u2010021\u201010125\u20106"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572\u2010019\u20100084\u20101"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00141.2022"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2022.101560"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.11.019"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms251910279"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCEP.116.004725"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/6067096"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.113970"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2018.09.036"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202001716"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.1c01006"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.11.042"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.114840"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202504493"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202303664"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2021.110239"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2012.150"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apmt.2023.102035"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201052221\u20109"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2021.109970"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2999"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.0c01422"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010024\u201002480\u20107"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/adbi.202000190"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.986107"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3233\/JND\u2010180314"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvad017"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967\u2010025\u201006349\u2010x"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/admt.202101636"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2018.02.007"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.8b01112"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2023.100626"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2020.120195"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2023.10.019"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758\u20105090\/acc42c"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.116.024145"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8BM01348A"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2021.03.097"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41378\u2010021\u201000344\u20100"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1311120110"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.87739.3"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1063\/5.0163363"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201062955\u20103"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44161\u2010024\u201000471\u20107"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.9b00052"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP274145"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2017.04.025"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2023.122255"},{"key":"e_1_2_9_54_1","first-page":"1","article-title":"Pathophysiology of Dilated Cardiomyopathy: From Mechanisms to Precision Medicine","volume":"22","author":"Gigli M.","year":"2024","journal-title":"Nature Reviews Cardiology"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3003552"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2013.09.003"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2015.09.010"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioadv.2022.212916"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000258172.74570.e6"},{"issue":"5","key":"e_1_2_9_60_1","first-page":"274","article-title":"\u03b2\u2010Adrenergic Regulation of Ca2+ Signaling in Heart Cells","volume":"10","author":"Yang B.","year":"2024","journal-title":"Biophysics Reports"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1111\/pace.13419"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.310083"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.304678"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.01453"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12551\u2010014\u20100152\u20104"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.13202"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2016.04.011"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.13\u2010228007"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1109\/JMEMS.2020.3011196"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010018\u20100016\u20103"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2016.09.024"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2025.117478"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2022.11.008"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201500427"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2020.0342"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41563\u2010021\u201000919\u20102"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41440\u2010022\u201000897\u20101"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compfluid.2019.104359"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12551\u2010024\u201001267\u20106"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2020.00811"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573\u2010020\u20100079\u20103"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crmeth.2026.101361"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1LC00241D"},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.1002\/adbi.202500536"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2019.06.005"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.3110"},{"key":"e_1_2_9_87_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.122.320496"},{"key":"e_1_2_9_88_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2024.1475152"},{"key":"e_1_2_9_89_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-026-70550-9"},{"key":"e_1_2_9_90_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvab075"},{"key":"e_1_2_9_91_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2023.100976"},{"key":"e_1_2_9_92_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41378\u2010026\u201001249\u20106"},{"key":"e_1_2_9_93_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2026.118717"},{"key":"e_1_2_9_94_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.118.311209"},{"key":"e_1_2_9_95_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.ado7089"},{"key":"e_1_2_9_96_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201013868\u2010x"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.71464","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.71464","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.71464","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T10:10:28Z","timestamp":1791540628000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.71464"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,25]]},"references-count":95,"journal-issue":{"issue":"37","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/adhm.71464"],"URL":"https:\/\/doi.org\/10.1002\/adhm.71464","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,25]]},"assertion":[{"value":"2025-12-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71464"}}