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The secreted extracellular vesicles (EVs) of organoids, in particular the small\u2010sized EV subset referred as exosomes (30\u2013200\u00a0nm), have emerged as novel therapeutics in regenerative medicine. This study investigated the encapsulation and controlled release of human spinal cord organoid (hSCO)\u2010derived EVs in viscoelastic hyaluronic acid (HA) hydrogels and assessed their impact on organoid patterning. A series of pH\u2010responsive hydrogels were fabricated, leading to sustained EV release regulated by viscoelastic properties. The pH of these hydrogels decreased from 9 to 7 during incubation, which altered hydrogel viscoelasticity, thereby modulating EV release kinetics. In addition, EV\u2010loaded hydrogels regulated key hSCO patterning markers such as\n                    <jats:italic>DBX1<\/jats:italic>\n                    and\n                    <jats:italic>ISL1<\/jats:italic>\n                    . Furthermore, these EVs in hydrogels can cross a modeled blood\u2013spinal cord barrier and provide cross\u2010barrier capability for delivery. Taken together, the organoid\u2010secreted EVs in viscoelastic HA hydrogels can be released at a controlled rate and have potential to regulate spinal cord organoid patterning. This study advances our knowledge of regulating intercellular communication and developing EV\u2010based therapies for treating neurological disorders such as spinal cord injury.\n                  <\/jats:p>","DOI":"10.1002\/adhm.71472","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T04:23:19Z","timestamp":1785730999000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Encapsulation and Controlled Release of Human Spinal Cord Organoid\u2010Derived Extracellular Vesicles for Tissue Patterning in Viscoelastic Hyaluronic Acid Hydrogels"],"prefix":"10.1002","volume":"15","author":[{"given":"Xingchi","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Chemical and Biomedical Engineering FAMU\u2010FSU College of Engineering Florida State University  Tallahassee Florida USA"},{"name":"Department of Industrial and Manufacture Engineering, FAMU\u2010FSU College of 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