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Based on the dual rationale of frequent lymph node metastasis in esophageal squamous cell carcinoma (ESCC) and prevalent TROP2 overexpression, the IRDye800cw\u2010labeled SG probe (SG\u2010IR800) was evaluated for detecting metastases of ESCC. Cellular uptake of SG was significantly higher in TROP2\u2010positive KYSE150 cells than in TROP2\u2010negative EC109 cells. Both subcutaneous KYSE150 tumors and patient\u2010derived xenograft models with high TROP2 expression demonstrated significantly higher accumulation of SG\u2010IR800 compared to the control probe. Peritoneal metastases yielded an NIR signal in the second window (NIR\u2010II) comparable to primary tumors, which outperformed the NIR\u2010I signal. Metastatic lymph nodes showed higher NIR\u2010II contrast than inflammatory nodes. This study demonstrates that the SG\u2010IR800 probe possesses high specificity for TROP2\u2010positive ESCC, facilitates the detection of metastatic lesions, and holds strong potential for guiding precise intraoperative navigation.<\/jats:p>","DOI":"10.1002\/adhm.71488","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:37:07Z","timestamp":1785285427000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Detection of Metastatic Lesions in Esophageal Cancer Using a TROP2 Antibody\u2010Based Near\u2010Infrared Probe"],"prefix":"10.1002","volume":"15","author":[{"given":"Huixiang","family":"Lan","sequence":"first","affiliation":[{"name":"Department of Radiology The Fifth Affiliated Hospital of Sun Yat\u2010Sen University  Zhuhai 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