{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T17:07:31Z","timestamp":1788887251467,"version":"build-2803163510"},"reference-count":84,"publisher":"Wiley","issue":"33","license":[{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Organoids are powerful models for developmental biology, disease modelling, and personalized medicine. However, their broader application is limited by the inability of extracellular matrix (ECM) hydrogels to mimic the biomechanical and biochemical properties of native tissues. Dependence on poorly\u2010defined basement membrane matrices such as Matrigel\u2014a xenogenic, tumor\u2010derived, nontunable material\u2014introduces organoid growth variability and constrains reproducibility across organoid systems. In this study, we report a fully defined ECM platform that integrates key biomechanical and biochemical features of native tissue to support organoid growth. Using gelatin methacryloyl (GelMA) as a tunable hydrogel backbone, supplemented with laminin\u2013entactin complex, we established a matrix with physiologically relevant stiffness and biochemical composition for mouse small intestinal organoids (mSIOs). The optimized matrix (2% w\/v DS95 GelMA with 2\u00a0mg mL\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    laminin\u2013entactin) supported robust mSIO proliferation, morphology, and differentiation comparable to or exceeding that achieved with Matrigel. Importantly, continuous passaging was achieved without affecting organoid quality, suggesting feasibility of long\u2010term mSIO expansion. These findings demonstrate that coordinated control of matrix mechanics and biochemical cues is critical for organoid development. The simplicity, stability, and tunability of this GelMA\u2010based system provides a scalable and reproducible alternative to Matrigel, with broad potential for organoid culture, mechanistic studies, and translational applications.\n                  <\/jats:p>","DOI":"10.1002\/adhm.71498","type":"journal-article","created":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T03:21:45Z","timestamp":1786159305000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Fully Defined GelMA\u2010Based Matrix Allows Fine Tuning of Tissue\u2010Relevant Biomechanical and Biochemical Cues for Organoid Culture"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-6020-3430","authenticated-orcid":false,"given":"Junyi","family":"Liu","sequence":"first","affiliation":[{"name":"Cancer Discovery and Regenerative Medicine, Lee Kong Chian School of Medicine Nanyang Technological University  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