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Although some chemical, physical, and synergetic technologies have been developed to treat malignant osteosarcoma, molecular mechanism of structural nanomechanics is indistinct, especially for inducement mechanism, malignant evaluation, and distant metastasis of osteosarcoma. We fabricated photo\u2010responsive biomaterials as the biofunctional UV\u2010mediated \u201ccell adhesion\u201d switch to construct the advanced microarrays on cell culture plates. The adhesion\u2010automatic microarrays were modified with fibronectin and osteosarcoma cells were micropatterned on the microarrays by microscopy and AFM observation. The focal adhesion (FA) was matured in microarrayed osteosarcoma cells by adhesion phase separation. The influence of phase\u2010separated FA\u2010induced cytoskeleton bias on spatial mechanical property and force heterogeneity is investigated to disclose its interaction with nuclear activity in microarrayed osteosarcoma cells. Heterogeneous force remodeling could monitor nuclear force\u2013sensing mechanotransduction, based on nuclear ectopia configuration by the evaluation of LaminA\/C, Ki67 and YAP analysis. This study will provide theoretical potential for understanding phase\u2010separated adhesion, heterogeneous mechanical remodeling, and nuclear force\u2013sensing mechanotransduction of malignant osteosarcoma.<\/jats:p>","DOI":"10.1002\/adhm.71508","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T04:27:04Z","timestamp":1785472024000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phase\u2010Separated Nanoarchitecture of Adhesion\u2010Cytoskeleton Force Coupling Reforms Nuclear Spatiotemporal Posture Conformation and Mechanotransduction of Osteosarcoma on Biofunctional Microarrays"],"prefix":"10.1002","volume":"15","author":[{"given":"Huayu","family":"Fan","sequence":"first","affiliation":[{"name":"Luoyang Orthopedic Hospital of Henan Province Orthopedic Hospital of Henan Province  Zhengzhou Henan 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