{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T02:41:50Z","timestamp":1792291310114,"version":"4.4.0"},"reference-count":30,"publisher":"Wiley","issue":"33","license":[{"start":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T00:00:00Z","timestamp":1786838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T00:00:00Z","timestamp":1786838400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7244334"],"award-info":[{"award-number":["7244334"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Organ preservation remains a critical challenge in transplantation, primarily due to hypothermia\u2010induced oxidative stress and metabolic dysfunction. Here, we report a mitochondria\u2010enriched, cell\u2010free preservation strategy by supplementing standard preservation solutions with freshly isolated mitochondria derived from human induced pluripotent stem cell\u2013mesenchymal stem cells (MSC\u2010mt). MSC\u2010mt retained intact ultrastructure and functional biophysical properties. In vitro, MSC\u2010mt were internalized by hepatocyte\u2010 and kidney\u2010derived cells, reduced oxidative stress, preserved ATP levels, and attenuated apoptosis under cold stress.\n                    <jats:italic>Ex vivo<\/jats:italic>\n                    , MSC\u2010mt improved liver preservation in University of Wisconsin (UW) solution, reducing sinusoidal edema, apoptosis, ALT\/AST release, MDA accumulation, and oxidative DNA damage while enhancing SOD activity and preserving mitochondrial content. Human\u2010specific mitochondrial signals remained detectable within preserved hepatic tissue. In a warm reoxygenation model, MSC\u2010mt enhanced ATP recovery and reduced tissue injury and oxidative damage following cold storage. In kidneys, MSC\u2010mt provided stronger protection than fibroblast\u2010 or adipose\u2010derived mitochondria across both HC\u2010A and UW solutions. Mechanistically, MSC\u2010mt showed higher total and phosphorylated PINK1 levels and greater Parkin co\u2010localization than fibroblast\u2010derived mitochondria, while mitophagy inhibition partially reversed their antioxidant effects. These findings establish MSC\u2010mt as a cell\u2010free mitochondrial strategy for improving hypothermic organ preservation.\n                  <\/jats:p>","DOI":"10.1002\/adhm.71510","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T04:28:53Z","timestamp":1786940933000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Stem Cell\u2010Derived Mitochondria\u2010Enriched Preservation Solutions as Organelle\u2010Based Therapeutic Strategy for Cold Storage of Liver and Kidney"],"prefix":"10.1002","volume":"15","author":[{"given":"Fang","family":"Lin","sequence":"first","affiliation":[{"name":"Translational Medical Center For Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East Hospital Tongji University School of Medicine  Shanghai China"},{"name":"Shanghai Heart Failure Research Center Shanghai East Hospital Tongji University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianxin","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Taizhou Fourth People's Hospital  Taizhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Burn and Plastic Surgery National Center for Children's Health Beijing Children's Hospital Capital Medical University  Xicheng Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8552-1381","authenticated-orcid":false,"given":"Yue","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Organ Transplantation Changzheng Hospital Naval Medical University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kexin","family":"Ma","sequence":"additional","affiliation":[{"name":"Translational Medical Center For Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East Hospital Tongji University School of Medicine  Shanghai China"},{"name":"Shanghai Heart Failure Research Center Shanghai East Hospital Tongji University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiyuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Organ Transplantation Changzheng Hospital Naval Medical University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0327-2549","authenticated-orcid":false,"given":"Xueyang","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Organ Transplantation Changzheng Hospital Naval Medical University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Organ Transplantation Changzheng Hospital Naval Medical University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Organ Transplantation Changzheng Hospital Naval Medical University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3262-1859","authenticated-orcid":false,"given":"Xiaoting","family":"Liang","sequence":"additional","affiliation":[{"name":"Translational Medical Center For Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East Hospital Tongji University School of Medicine  Shanghai China"},{"name":"Shanghai Heart Failure Research Center Shanghai East Hospital Tongji University School of Medicine  Shanghai China"},{"name":"Research Center for Translational Medicine Shenzhen Ruipuxun Academy for Stem Cell and Regenerative Medicine  Shenzhen 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