{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T08:16:44Z","timestamp":1788509804928,"version":"build-2803163510"},"reference-count":51,"publisher":"Wiley","issue":"33","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Human induced pluripotent stem cell\u2010derived kidney organoids represent promising in vitro models for studying kidney development and drug\u2010induced toxicity, yet their lack of vasculature limits maturation and translational use. Here, we present a proof\u2010of\u2010concept co\u2010culture platform to promote vascularization of kidney organoids by integrating stromal vascular fraction cells, collagen sponge scaffolds, porcine kidney decellularized extracellular matrix (dECM), and dynamic culture in a perfusion\u2010based U\u2010CUP bioreactor. We systematically evaluated the effects of serum supplementation, scaffold pre\u2010vascularization, dECM encapsulation, and flow conditions on organoid\u2013vascular integration. Low\u2010level serum supplementation (1.5% FBS) sustained vascular networks without compromising organoid morphology. Perfusion supported vascular expansion; however, while a low flow rate comparable to interstitial tissue flow (0.01\u00a0mL\/min) preserved organoid morphology and glomerular\u2010like structures, a higher flow rate optimized for vascularization (0.47\u00a0mL\/min) disrupted overall organoid architecture. Encapsulation within dECM preserved glomerular morphology and supported vascular infiltration, whereas co\u2010culture with stromal vascular fraction cells enabled putative podocyte\u2013endothelial interactions. Finally, exposure to the calcineurin inhibitor tacrolimus revealed dose\u2010dependent cytotoxicity and characteristic nephron and vascular injury, demonstrating the model's utility for nephrotoxicity screening. The established modular platform enables the engineering of vascularized kidney organoids, establishing foundations for physiologically relevant models for disease modeling and pre\u2010clinical drug testing.<\/jats:p>","DOI":"10.1002\/adhm.71526","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T06:36:58Z","timestamp":1786430218000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Vascularization of Human iPSC\u2010Derived Kidney Organoids Using Perfusion Culture, Pre\u2010Vascularized Collagen Scaffolds, and Decellularized Extracellular Matrix"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9901-3221","authenticated-orcid":false,"given":"Helen","family":"Kearney","sequence":"first","affiliation":[{"name":"MERLN Institute for Technology\u2010Inspired Regenerative Medicine Maastricht University  Maastricht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1486-8011","authenticated-orcid":false,"given":"Andrea","family":"Mazzoleni","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6493-0432","authenticated-orcid":false,"given":"Ivan","family":"Martin","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Basel  Basel Switzerland"},{"name":"Tissue Engineering Department of Biomedicine University of Basel and University Hospital of Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1298-6025","authenticated-orcid":false,"given":"Lorenzo","family":"Moroni","sequence":"additional","affiliation":[{"name":"MERLN Institute for Technology\u2010Inspired Regenerative Medicine Maastricht University  Maastricht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4590-1916","authenticated-orcid":false,"given":"Manuele Giuseppe","family":"Muraro","sequence":"additional","affiliation":[{"name":"Tissue Engineering Department of Biomedicine University of Basel and University Hospital of Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5935-6245","authenticated-orcid":false,"given":"Carlos","family":"Mota","sequence":"additional","affiliation":[{"name":"MERLN Institute for Technology\u2010Inspired Regenerative Medicine Maastricht University  Maastricht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,10]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.4137\/BMI.S20054"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature15695"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2017.10.011"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3392"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2018.06.018"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2018.10.010"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology12040503"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2019.06.001"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2018.01.041"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0325-y"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/adb4a1"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/ad5ac0"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-24945-5"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-13882-3"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202103526"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.113.302598"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.04.064"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.170"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-025-10971-z"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202400807"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_12_23_1","first-page":"299","article-title":"Kidney Injury Molecule\u20101: A Translational Journey","volume":"125","author":"Bonventre J. V.","year":"2014","journal-title":"Transactions of the American Clinical and Climatological Association"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abj4772"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2004.08.002"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41698-025-00941-6"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202501416"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00400.2003"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cdev.2021.203735"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2010.05.001"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2009.108"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-019-03401-0"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44385-025-00063-5"},{"issue":"1","key":"e_1_2_12_34_1","first-page":"132","article-title":"Morphological Process of Podocyte Development Revealed by Block\u2010Face Scanning Electron Microscopy","volume":"130","author":"Ichimura K.","year":"2017","journal-title":"Journal of Cell Science"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00069.2020"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2012.02.031"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202400306"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2007.00031.x"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41563-019-0287-6"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.3904\/kjim.2020.323"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/142521"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.04800908"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/aor.13551"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2005.01047.x"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.14190"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700215"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2596"},{"issue":"11","key":"e_1_2_12_48_1","article-title":"Beyond Immunosuppression: The Intricate Relationship Between Tacrolimus and Microangiopathy","volume":"15","author":"Munjal R. S.","year":"2023","journal-title":"Cureus"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaccas.2025.103790"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1159\/000330187"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202500301"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crmeth.2024.100779"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.71526","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.71526","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.71526","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T07:49:17Z","timestamp":1788508157000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.71526"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,10]]},"references-count":51,"journal-issue":{"issue":"33","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/adhm.71526"],"URL":"https:\/\/doi.org\/10.1002\/adhm.71526","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,10]]},"assertion":[{"value":"2026-03-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71526"}}